A molecular test to identify resistance alleles at the amplified esterase locus in the mosquito Culex pipiens

被引:0
|
作者
Berticat, C [1 ]
Dubois, MP [1 ]
Marquine, M [1 ]
Chevillon, C [1 ]
Raymond, M [1 ]
机构
[1] Univ Montpellier 2, Inst Sci Evolut, CNRS,UMR 5554, Lab Genet & Environm,Team Genet Adaptat, F-34095 Montpellier 05, France
关键词
esterase; insecticide resistance gene; molecular test; mosquito; Culex pipiens;
D O I
10.1002/1526-4998(200009)56:9<727::AID-PS214>3.3.CO;2-9
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
A new PCR/RFLP method is presented to identify all described alleles involved in resistance at the Ester locus in the mosquito Culex pipiens. The efficiency of this method as well as its advantages compared with the traditional identification technique (starch gel electrophoresis) have been tested in four natural samples from France, Tunisia and California. This simple and fast molecular test is a very convenient tool for studies in field populations and laboratory strains. (C) 2000 Society of Chemical Industry.
引用
收藏
页码:727 / 731
页数:5
相关论文
共 50 条
  • [1] Recombination between two amplified esterase alleles in Culex pipiens
    Berticat, C
    Marquine, M
    Raymond, M
    Chevillon, C
    JOURNAL OF HEREDITY, 2001, 92 (04) : 349 - 351
  • [2] Evolution of insecticide resistance in the mosquito Culex pipiens: The migration hypothesis of amplified esterase genes
    Raymond, M
    Pasteur, N
    MOLECULAR GENETICS AND EVOLUTION OF PESTICIDE RESISTANCE, 1996, 645 : 90 - 96
  • [3] INHERITANCE OF AN ESTERASE LOCUS IN CULEX-PIPIENS
    GARGAN, TP
    BARR, AR
    ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA, 1977, 70 (03) : 402 - 408
  • [4] Dynamics of Esterase Alleles in Culex pipiens Complex Mosquitoes in Beijing
    Yan, Shuaiguo
    Wu, Zhonghua
    Cui, Feng
    Zhao, Qiao
    Qiao, Chuanling
    JOURNAL OF ECONOMIC ENTOMOLOGY, 2008, 101 (06) : 1897 - 1902
  • [5] Esterase polymorphism in insecticide susceptible populations of the mosquito Culex pipiens
    Raymond, M
    Qiao, CL
    Callaghan, A
    GENETICAL RESEARCH, 1996, 67 (01) : 19 - 26
  • [6] Distribution and dynamics of esterase alleles in Culex pipiens complex in China
    Yan, Shuaiguo
    Nan, Ping
    Cui, Feng
    Wu, Zhonghua
    Qiao, Chuanling
    JOURNAL OF ASIA-PACIFIC ENTOMOLOGY, 2013, 16 (01) : 43 - 48
  • [8] Tracking the evolution of insecticide resistance in the mosquito Culex pipiens
    Thomas Lenormand
    Denis Bourguet
    Thomas Guillemaud
    Michel Raymond
    Nature, 1999, 400 : 861 - 864
  • [9] Tracking the evolution of insecticide resistance in the mosquito Culex pipiens
    Lenormand, T
    Bourguet, D
    Guillemaud, T
    Raymond, M
    NATURE, 1999, 400 (6747) : 861 - 864
  • [10] Population genetics of insecticide resistance in the mosquito Culex pipiens
    Chevillon, C
    Raymond, M
    Guillemaud, T
    Lenormand, T
    Pasteur, N
    BIOLOGICAL JOURNAL OF THE LINNEAN SOCIETY, 1999, 68 (1-2) : 147 - 157