Genome-Wide Survey and Expression Analyses of Hexokinase Family in Poplar (Populus trichocarpa)

被引:4
|
作者
Han, Mei [1 ]
Xu, Xianglei [1 ,2 ]
Xiong, Yuan [1 ,2 ]
Wei, Haikun [1 ]
Yao, Kejun [1 ]
Huang, Tingting [1 ]
Long, Yingle [1 ]
Su, Tao [1 ,2 ]
机构
[1] Nanjing Forestry Univ, Coll Biol & Environm, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing 210037, Peoples R China
[2] Nanjing Forestry Univ, Key Lab State Forestry Adm Subtrop Forest Biodive, Nanjing 210037, Peoples R China
来源
PLANTS-BASEL | 2022年 / 11卷 / 15期
基金
中国国家自然科学基金;
关键词
Populus; hexokinase; sucrose metabolism; sugar signaling; stress and defense; ARABIDOPSIS HEXOKINASE; STRUCTURAL-ANALYSIS; GENE FAMILY; GLUCOSE; SUGAR; PLANTS; MYOINOSITOL; PHYSIOLOGY; STRESS; GROWTH;
D O I
10.3390/plants11152025
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Hexokinase (HXK) family proteins exert critical roles in catalyzing hexose phosphorylation, sugar sensing, and modulation of plant growth and stress adaptation. Nevertheless, a large amount remains unknown about the molecular profile of HXK enzymes in Populus trichocarpa, a woody model tree species. A genome-wide survey of HXK-encoding genes, including phylogenies, genomic structures, exon/intron organization, chromosomal distribution, and conserved features, was conducted, identifying six putative HXK isogenes (PtHXK1-6) in the Populus genome. The evolutionary tree demonstrated that 135 homologous HXKs between 17 plant species were categorized into four major subfamilies (type A, B, C, and D), clustering one plastidic (PtHXK3) and five mitochondrial PtHXKs grouped into type A and B, respectively. The in silico deduction prompted the presence of the conserved sugar-binding core (motif 4), phosphorylation sites (motif 2 and 3), and adenosine-binding domains (motif 7). The transcriptomic sequencing (RNA-seq) and the quantitative real-time PCR (qRT-PCR) assays revealed that three isogenes (PtHXK2, 3, and 6) were abundantly expressed in leaves, stems, and roots, while others appeared to be dominantly expressed in the reproductive tissues. Under the stress exposure, PtHXK2 and 6 displayed a significant induction upon the pathogenic fungi (Fusarium solani) infection and marked promotions by glucose feeding in roots. In contrast, the PtHXK3 and 6 are ABA-responsive genes, following a dose-dependent manner. The comprehensive analyses of the genomic patterns and expression profiling provide theoretical clues and lay a foundation for unraveling the physiological and signaling roles underlying the fine-tuned PtHXKs responding to diverse stressors.
引用
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页数:16
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