Gold Nanoparticles Modified a Multimode Clad-Free Fiber for Ultrasensitive Detection of Bovine Serum Albumin

Gold nanoparticles (Au NPs) were almost chosen as the first option for biological and biosensor applications due to their enhancement and their outstanding properties. The combining of optical fiber with localized surface plasmon resonance (LSPR) for forming a biosensor is widely used in diagnosis. In this work, we report a fiber optical biosensor based on LSPR of Au NPs for the detection of bovine serum albumin (BSA) protein. BSA was functionalized on Au NPs immobilized fiber optic sensing head (length of 1 cm) via methanesulfonic acid (MSA) by carboxylic binding. It is the binding between the analytes with the surface-modified Au NPs that caused refractive index changes in the sensing medium led to changes in optical power at the output of the sensor. The detection limit of the LSPR fiber biosensor was found to be 0.18 ng/mL for the BSA detection with the low coefficient of variation (CV) at under 1%. We have demonstrated the effectiveness of combining multimode fiber with Au NPs to generate the biosensor as the label-free sensor that can be a feasible tool for highly sensitive, rapid response time, stable, and miniaturized point-of-care analytical systems.