Imidazolium-tagged glycan probes for non-covalent labeling of live cells

被引：11
|
作者：
Benito-Alifonso, David [1 ]
Tremell, Shirley [1 ]
Sadler, Joanna C. [1 ]
Berry, Monica [2 ]
Galan, M. Carmen [1 ]
机构：
[1] Univ Bristol, Sch Chem, Cantocks Close, Bristol BS8 1TS, Avon, England
[2] Univ Bristol, NSQI, Sch Phys, Tyndall Ave, Bristol BS8 1F, Avon, England
来源：
CHEMICAL COMMUNICATIONS | 2016年 / 52卷 / 27期
基金：
英国工程与自然科学研究理事会; 欧洲研究理事会;
关键词：
SURFACE SIALIC ACIDS; CHEMICAL REPORTERS; OLIGOSACCHARIDE SYNTHESIS; CHEMOENZYMATIC SYNTHESIS; MODIFIED PROTEINS; CLICK CHEMISTRY; LIVING ANIMALS; IONIC LIQUIDS; GLYCOSYLATION; ISONITRILE;
D O I：
10.1039/c5cc10040b
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Selective, bioorthogonal and fast labeling of glycoconjugates in living cells is a major challenge for synthetic and cellular biology. Here we report the use imidazolium tagged-mannosamine derivative (ITag-Man) for the non-covalent, rapid and site-specific labeling of sialic acid containing glycoproteins using commercial N-nitrilotriacetate fluorescent reagents in a range of cell lines.
引用
收藏
页码：4906 / 4909
页数：4
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