Imaging of Surfaces by Concurrent Surface Plasmon Resonance and Surface Plasmon Resonance-Enhanced Fluorescence

被引：6
|
作者：
Thariani, Rahber [1 ]
Yager, Paul [1 ]
机构：
[1] Univ Washington, Dept Bioengn, Seattle, WA 98195 USA
来源：
PLOS ONE | 2010年 / 5卷 / 03期
基金：
比尔及梅琳达.盖茨基金会; 美国国家卫生研究院;
关键词：
SPECTROSCOPY; IMMUNOASSAY; MICROSCOPE; KINETICS; HEALTH; ADSORPTION; DEPENDENCE; FILMS; ACID; CARE;
D O I：
10.1371/journal.pone.0009833
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Surface plasmon resonance imaging and surface plasmon induced fluorescent are sensitive tools for surface analysis. However, existing instruments in this area have provided limited capability for concurrent detection, and may be large and expensive. We demonstrate a highly cost-effective system capable of concurrent surface plasmon resonance microscopy (SPRM) and surface plasmon resonance-enhanced fluorescence (SPRF) imaging, allowing for simultaneous monitoring of reflectivity and fluorescence from discrete spatial regions. The instrument allows for high performance imaging and quantitative measurements with surface plasmon resonance, and surface plasmon induced fluorescence, with inexpensive off-the-shelf components.
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页数：4
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