Mutations in lysX as the new and reliable markers for tuberculosis Beijing and modern Beijing strains

被引：0
|
作者：
Zhao, Li-li [1 ]
Xiao, Tong-yang [1 ]
Sun, Qing [1 ,2 ]
Liu, Hai-can [1 ]
Zhao, Xiu-qin [1 ]
Jiang, Yi [1 ]
Li, Gui-lian [1 ]
Zeng, Chun-yan [3 ]
Wan, Kang-lin [1 ,2 ]
机构：
[1] Chinese Ctr Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Collaborat Innovat Ctr Diag & Treatment Infect Di, Natl Inst Communicable Dis Control & Prevent, Beijing 102206, Peoples R China
[2] Hunan Tradit Chinese Med Coll, Dept Med, Zhuzhou 412012, Peoples R China
[3] Hulunbeier Peoples Hosp, Hulunbeier 021000, Peoples R China
来源：
TUBERCULOSIS | 2016年 / 97卷
基金：
中国国家自然科学基金;
关键词：
Mycobacterium tuberculosis; Beijing genotype; Modern Beijing subtype; Marker; Genotype; Multiplex allele-specific PCR; MYCOBACTERIUM-TUBERCULOSIS; GENETIC DIVERSITY; MUTT GENES; FAMILY; GENOTYPE; LINEAGE; TRANSMISSION; SUBLINEAGE; ORIGIN; PCR;
D O I：
10.1016/j.tube.2015.12.006
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Genotyping results and DNA sequencing analysis of 235 Mycobacterium tuberculosis (M. tuberculosis) isolates from China indicated that mutations at codon 995 (Pro CCG to Pro CCA) and 701 (Ile ATT to Thr ACT) in lysX gene (Rv1640c), are specific markers for Beijing and modern Beijing strains, respectively. This observation was also confirmed by 24 genomes of M. tuberculosis strains from other countries. Moreover, a simple and fast multiplex allele-specific PCR (MAS-PCR) method for detecting mutations at codon 995 and 701 in lysX has been established and used to screen 235 DNA samples obtained from M. tuberculosis isolates. In all cases, Beijing and modern Beijing strains were identified correctly. (c) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码：33 / 37
页数：5
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