Ablation of plasma membrane Ca2+-ATPase isoform 4 prevents development of hypertrophy in a model of hypertrophic cardiomyopathy

被引:8
|
作者
Prasad, Vikram [1 ]
Lorenz, John N. [2 ]
Lasko, Valerie M. [2 ]
Nieman, Michelle L. [2 ]
Jiang, Min [3 ]
Gao, Xu [3 ]
Rubinstein, Jack [3 ]
Wieczorek, David F. [1 ]
Shull, Gary E. [1 ]
机构
[1] Univ Cincinnati, Coll Med, Dept Mol Genet Biochem & Microbiol, Cincinnati, OH 45267 USA
[2] Univ Cincinnati, Coll Med, Dept Mol & Cellular Physiol, Cincinnati, OH 45267 USA
[3] Univ Cincinnati, Coll Med, Dept Internal Med, Cincinnati, OH 45267 USA
基金
美国国家卫生研究院;
关键词
Hypertrophic cardiomyopathy; PMCA4; Pathological hypertrophy; Diastolic dysfunction; Ventricular stiffness; Diastolic properties; SARCOLEMMAL CALCIUM-PUMP; NITRIC-OXIDE SYNTHASE; PHOSPHOPROTEIN PHOSPHATASE 2A; SEVERE CARDIAC-HYPERTROPHY; HEART-FAILURE; DILATED CARDIOMYOPATHY; CATALYTIC SUBUNIT; DIASTOLIC DYSFUNCTION; CARBOXYL METHYLATION; PRESSURE-OVERLOAD;
D O I
10.1016/j.yjmcc.2014.09.025
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The mechanisms linking the expression of sarcomeric mutant proteins to the development of pathological hypertrophy in hypertrophic cardiomyopathy (HCM) remain poorly understood. We investigated the role of the plasma membrane Ca2+-ATPase PMCA4 in the HCM phenotype using a transgenic model that expresses mutant (Glu180Gly) alpha-tropomyosin (Tm180) in heart. Immunoblot analysis revealed that cardiac PMCA4 expression was upregulated early in Tm180 disease pathogenesis. This was accompanied by an increase in levels of the L-type Ca2+-channel, which is implicated in pathological hypertrophy. When Tm180 mice were crossed with a PMCA4-null line, loss of PMCA4 caused the abrogation of hypertrophy in Tm180/PMCA4-null double mutant mice. RT-PCR analysis of Tm180/PMCA4-null hearts revealed blunting of the fetal program and reversion of pro-fibrotic Col1a1 and Col3a1 gene expression to wild-type levels. This was accompanied by evidence of reduced L-type Ca2+-channel expression, and diminished calcineurin activity. Expression of the metabolic substrate transporters glucose transporter 4 and carnitine palmitoyltransferase 1b was preserved and Tm180-related changes in mRNA levels of various contractile stress-related proteins including the cardiac ankyrin protein CARP and the N2B isoform of titin were reversed in Tm180/PMCA4-null hearts. cGMP levels were increased and phosphorylation of vasodilator-stimulated phosphoprotein was elevated in Tm180/PMCA4-null hearts. These changes were associated with a sharp reduction in left ventricular end-diastolic pressure in Tm180/PMCA4-null hearts, which occurred despite persistence of Tm180-related impairment of relaxation dynamics. These results reveal a novel and specific role for PMCA4 in the Tm180 hypertrophic phenotype, with the "protective" effects of PMCA4 deficiency encompassing multiple determinants of HCM-related hypertrophy. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码:53 / 63
页数:11
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