Molecular characterization of virulent isolates of root-knot nematode, Meloidogyne incognita by using RAPD-PCR

The selected six indigenous virulent isolates of Meloidogyne incognita subjected to RAPD analysis revealed that out of 12 random primers screened, three random primers showed reproducible amplification product for all the six isolates. The amplified product of three random primers viz., OPA-01, OPA-11 and OPA-13 showed considerable degree of polymorphism between them. Based on the presence and absence of the amplification products, the Squared Euclidean Distances as well as Dendrograrn were constructed. Two clusters were clearly illustrated. The polymorphism among the isolates was 90.19%. The average number of bands per primer was 17, while the average number of polymorphic bands per primer was 15.33.