Parasphingorhabdus halotolerans sp. nov. isolated from marine sediment in Jeju Island

A Gram-stain-negative, catalase- and oxidase-positive, rod-shaped bacterium, designated as JK6(T) was isolated from a coastal marine sediment in Jeju Island. Strain JK6(T) was characterized by polyphasic investigation including genome features. It grew at pH 5.0-9.0 (optimum 7.5), 18-30 degrees C (optimum 25 degrees C) and 1.0-7.0% (w/v) NaCl (optimum 2.0%). Strain JK6(T) utilized d-mannose, d-glucose, l-fucose, propionate and acetate as carbon and energy sources. The sole quinone was ubiquinone-10, and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, and sphingolipid. Strain JK6(T) was closely related to Parasphingorhabdus flavimaris SW-151(T) (98.2%), Parasphingorhabdus marina DSM 22363(T) (97.6%) and Parasphingorhabdus litoris FR1093(T) (97.6%) based on 16S rRNA gene sequence similarity. Genome length and GC content were 3.29 Mbp and 53.0%, respectively. Digital DNA-DNA relatedness, average nucleotide identity, and average amino acid identity between strain JK6(T) and P. flavimaris SW-151(T) were 16.6%, 73.9%, and 77.6%, respectively. These results showed that the strain can be recognized as a novel bacterium named Parasphingorhabdus halotolerans. The type strain of Parasphingorhabdus halotolerans sp. nov. is JK6(T) (= KCTC 72818(T) = VTCC 910111(T)).