A novel one-step amplification refractory mutation system PCR (ARMS-PCR) for differentiation of canine parvovirus-2 variants

被引:6
|
作者
Dema, Anusha [1 ]
Ganji, Vishweshwar Kumar [1 ]
Yella, Narasimha Reddy [2 ]
Putty, Kalyani [1 ]
机构
[1] PVNRTVU, Coll Vet Sci, Dept Vet Biotechnol, Hyderabad 500030, India
[2] PVNRTVU, Coll Vet Sci, Dept Vet Microbiol, Hyderabad 500030, India
关键词
CPV-2; ARMS-PCR; Antigenic typing; Phylogenetic analysis; POLYMERASE-CHAIN-REACTION; PARTIAL VP2 GENE; PHYLOGENETIC ANALYSIS; MOLECULAR CHARACTERIZATION; TYPE-2; EPIDEMIOLOGY; REPLACEMENT; EVOLUTION; DIAGNOSIS; SEQUENCE;
D O I
10.1007/s11262-021-01861-w
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Enteritis caused by CPV-2 antigenic variants (CPV-2a, 2b, and 2c) is frequently reported in dogs worldwide leading to significant morbidity and mortality. Here, we describe about a simple, single-step, ARMS-PCR strategy targeting the mutant 426 amino acid of VP2 to differentiate CPV-2 antigenic types. A total of 150 fecal samples were subjected to ARMS-PCR of which 18 were typed as CPV-2a, 79 were typed as CPV-2b, and 6 were typed as CPV-2c. The ARMS-PCR results were validated by randomly sequencing partial VP2 gene of 14 samples. Phylogenetic analysis of partial VP2 gene sequencing of each of the CPV-2 variants revealed that CPV-2a and CPV-2b isolates formed a separate clade of Indian lineage, while CPV-2c shared common evolutionary origin with Asian lineage. The developed technique is first of its kind, one-step, rapid, sequencing independent method for typing of CPV-2 antigenic variants.
引用
收藏
页码:426 / 433
页数:8
相关论文
共 50 条
  • [31] Rapid and efficient identification of the mouse leptin receptor mutation (C57BL/KsJ-db/db) by tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) analysis
    Harry Jung
    Hajin Nam
    Jun-Gyo Suh
    Laboratory Animal Research, 2016, 32 (1) : 70 - 73
  • [32] Erratum to: Rapid and efficient identification of the mouse leptin receptor mutation (C57BLKS/J-Leprdb) by tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) analysis
    Harry Jung
    Hajin Nam
    Jun-Gyo Suh
    Laboratory Animal Research, 2016, 32 (2) : 133 - 133
  • [33] Tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) rapidly identified a critical missense mutation (P236T) of bovine ACADVL gene affecting growth traits
    Zhang, Sihuan
    Dang, Yonglong
    Zhang, Qingfeng
    Qin, Qiaomei
    Lei, Chuzhao
    Chen, Hong
    Lan, Xianyong
    GENE, 2015, 559 (02) : 184 - 188
  • [34] Development of a novel one-step triplex PCR assay for the simultaneous detection of porcine circovirus type 2, porcine parvovirus and classical swine fever virus in a single tube
    Rajkhowa, S.
    Choudhury, M.
    Pegu, S. R.
    Sarma, D. K.
    Gupta, V. K.
    LETTERS IN APPLIED MICROBIOLOGY, 2022, 75 (02) : 338 - 344
  • [35] Ultrasensitive Amplification Refractory Mutation System Real-Time PCR (ARMS RT-PCR) Assay for Detection of Minority Hepatitis B Virus-Resistant Strains in the Era of Personalized Medicine
    Ntziora, Fotinie
    Paraskevis, Dimitrios
    Haida, Catherine
    Manesis, Emanuel
    Papatheodoridis, George
    Manolakopoulos, Spilios
    Elefsiniotis, Ioannis
    Karamitros, Timokratis
    Vassilakis, Alexis
    Hatzakisa, Angelos
    JOURNAL OF CLINICAL MICROBIOLOGY, 2013, 51 (09) : 2893 - 2900
  • [36] Developing an Amplification Refractory Mutation System-Quantitative Reverse Transcription-PCR Assay for Rapid and Sensitive Screening of SARS-CoV-2 Variants of Concern
    Xiong, Dongyan
    Zhang, Xiaoxu
    Shi, Mengjuan
    Wang, Nuo
    He, Ping
    Dong, Zhuo
    Zhong, Jie
    Luo, Jing
    Wang, Yong
    Yu, Junping
    Wei, Hongping
    MICROBIOLOGY SPECTRUM, 2022, 10 (01):
  • [37] Fast detection of SARS-CoV-2 variants including Omicron using one-step RT-PCR and Sanger sequencing
    Bloemen, Mandy
    Rector, Annabel
    Swinnen, Jill
    Van Ranst, Marc
    Maes, Piet
    Vanmechelen, Bert
    Wollants, Elke
    JOURNAL OF VIROLOGICAL METHODS, 2022, 304
  • [38] Detection and quantification of heteroplasmic mutant mitochondrial DNA by real-time amplification refractory mutation system quantitative PCR analysis: A single-step approach
    Bai, RK
    Wong, LJC
    CLINICAL CHEMISTRY, 2004, 50 (06) : 996 - 1001
  • [39] Specific Detection of SARS-CoV-2 Variants B.1.1.7 (Alpha) and 8.1.617.2 (Delta) Using a One-Step Quantitative PCR Assay
    Erster, Oran
    Mendelson, Ella
    Kabat, Areej
    Levy, Virginia
    Mannasse, Batya
    Assraf, Hadar
    Azar, Roberto
    Ali, Yaniv
    Bucris, Efrat
    Bar-Ilan, Dana
    Mor, Orna
    Elul, Michal
    Mandelboim, Michal
    Sofer, Danit
    Fleishon, Shay
    Zuckerman, Neta S.
    Bar-Or, Itay
    MICROBIOLOGY SPECTRUM, 2022, 10 (02):
  • [40] A sensitive one-step real-time RT-PCR method for detecting Grapevine leafroll-associated virus 2 variants in grapevine
    Beuve, Monique
    Sempe, Lauriane
    Lemaire, Olivier
    JOURNAL OF VIROLOGICAL METHODS, 2007, 141 (02) : 117 - 124