Rapid Method for Identification of Six Common Species of Mycobacteria Based on Multiplex SNP Analysis

被引：9
|
作者：
Wang, Hongxiu [1 ]
Yue, Jun [1 ]
Han, Min [1 ]
Yang, Jinghui [1 ]
Zhao, Yanlin [2 ]
机构：
[1] Tongji Univ, Shanghai Pulm Hosp, Key Lab Mycobacteria TB, Shanghai 200433, Peoples R China
[2] Beijing Thorac Tumor & TB Res Inst, TB Reference Lab, Beijing 101149, Peoples R China
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2010年 / 48卷 / 01期
关键词：
NONTUBERCULOUS MYCOBACTERIA; PROBE ASSAY; DIFFERENTIATION; MUTATIONS; INFECTION; SPECIMENS; DISEASE; SYSTEM; HIV; DNA;
D O I：
10.1128/JCM.01084-09
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A multiplex method using the SNaPshot technique was developed to screen for six common mycobacterial species: Mycobacteria tuberculosis, M. avium, M. intracellulare, M. chelonae, M. kansasii, and M. gordonae. A total of 468 mycobacterial clinical isolates were subjected to analysis for the presence of the six mycobacterial species by the multiplex SNaPshot method. Of the 468 mycobacterial isolates, 464 (99.15%) could be correctly identified by this assay. The multiplex SNaPshot technique is a promising discriminatory tool for rapid and accurate identification of frequently encountered clinical mycobacterial species.
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页码：247 / 250
页数：4
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