Combined virus-like particle and fusion protein-encoding DNA vaccination of cotton rats induces protection against respiratory syncytial virus without causing vaccine-enhanced disease

被引：26
|
作者：
Hwang, Hye Suk [1 ,2 ]
Lee, Young-Tae [1 ,2 ]
Kim, Ki-Hye [1 ,2 ]
Park, Soojin [1 ,2 ]
Kwon, Young-Man [1 ,2 ]
Lee, Youri [1 ,2 ]
Ko, Eun-Ju [1 ,2 ]
Jung, Yu-Jin [1 ,2 ]
Lee, Jong Seok [1 ,2 ,3 ]
Kim, Yu-Jin [1 ,2 ]
Lee, Yu-Na [1 ,2 ,4 ]
Kim, Min-Chul [1 ,2 ,4 ]
Cho, Minkyoung [1 ,2 ]
Kang, Sang-Moo [1 ,2 ]
机构：
[1] Georgia State Univ, Inst Biomed Sci, Ctr Inflammat Immun & Infect, Atlanta, GA 30303 USA
[2] Georgia State Univ, Dept Biol, Atlanta, GA 30303 USA
[3] Natl Inst Biol Resources, Inchon, South Korea
[4] Anim & Plant Quarantine Agcy, Gimcheon, Gyeongsangbukdo, South Korea
来源：
VIROLOGY | 2016年 / 494卷
关键词：
Respiratory syncytial virus; Safety; Cotton rats; Virus-like particle; Protection; PULMONARY HISTOPATHOLOGY; INTERLEUKIN-4; IL-4; RSV CHALLENGE; BALB/C MICE; T-CELLS; INFECTION; IMMUNIZATION; GLYCOPROTEIN; IMMUNOPATHOLOGY; NANOPARTICLE;
D O I：
10.1016/j.virol.2016.04.014
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A safe and effective vaccine against respiratory syncytial virus (RSV) should confer protection without causing vaccine-enhanced disease. Here, using a cotton rat model, we investigated the protective efficacy and safety of an RSV combination vaccine composed of F-encoding plasmid DNA and virus-like particles containing RSV fusion (F) and attachment (G) glycoproteins (FFG-VLP). Cotton rats with FFG-VLP vaccination controlled lung viral replication below the detection limit, and effectively induced neutralizing activity and antibody-secreting cell responses. In comparison with formalin inactivated RSV (FI-RSV) causing severe RSV disease after challenge, FFG-VLP vaccination did not cause weight loss, airway hyper responsiveness, IL-4 cytokines, histopathology, and infiltrates of proinflammatory cells such as eosinophils. FFG-VLP was even more effective in preventing RSV-induced pulmonary inflammation than live RSV infections. This study provides evidence that FFG-VLP can be developed into a safe and effective RSV vaccine candidate. (C) 2016 Published by Elsevier Inc.
引用
收藏
页码：215 / 224
页数：10
相关论文
共 32 条
  • [31] Respiratory syncytial virus (RSV) fusion protein expressed by recombinant Sendai virus elicits B-cell and T-cell responses in cotton rats and confers protection against RSV subtypes A and B
    Zhan, Xiaoyan
    Hurwitz, Julia L.
    Krishnamurthy, Sateesh
    Takimoto, Toru
    Boyd, Kelli
    Scroggs, Ruth A.
    Surman, Sherri
    Portner, Allen
    Slobod, Karen S.
    VACCINE, 2007, 25 (52) : 8782 - 8793
  • [32] Nervous Necrosis Virus-like Particle (VLP) Vaccine Stimulates European Sea Bass Innate and Adaptive Immune Responses and Induces Long-Term Protection against Disease
    Barsoe, Sofie
    Skovgaard, Kerstin
    Sepulveda, Dagoberto
    Stratmann, Ansgar
    Vendramin, Niccolo
    Lorenzen, Niels
    PATHOGENS, 2021, 10 (11):
1234