Localization of NMDA receptor subunits and mapping NMDA drive within the mammalian retina

被引:56
|
作者
Kalloniatis, M
Sun, D
Foster, L
Haverkamp, S
Wässle, H
机构
[1] Univ Auckland, Dept Optometry & Vis Sci, Auckland 1, New Zealand
[2] Univ Melbourne, Dept Optometry & Vis Sci, Melbourne, Vic, Australia
[3] Max Planck Inst Hirnforsch, D-60528 Frankfurt M, Germany
关键词
glutamate; NR1; NR1C2; NR2A; NR2B; agmatine; L-AP4;
D O I
10.1017/S0952523804214080
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Glutamate is a major neurotransmitter in the retina and other parts of the central nervous system, exerting its influence through ionotropic and metabotropic receptors. One ionotropic receptor, the N-methyl-D-aspartate (NMDA) receptor, is central to neural shaping, but also plays a major role during neuronal development and in disease processes. We studied the distribution pattern of different subunits of the NMDA receptor within the rat retina including quantifying the pattern of labelling for all the NR1 splice variants, the NR2A and NR2B subunits. The labelling pattern for the subunits was confined predominantly in the outer two-thirds of the inner plexiform layer. We also wanted to probe NMDA receptor function using an organic cation, agmatine (AGB); a marker for cation channel activity. Although there was an NMDA concentration-dependent increase in AGB labelling of amacrine cells and ganglion cells, we found no evidence of functional NMDA receptors on horizontal cells in the peripheral rabbit retina, nor in the visual streak where the type A horizontal cell was identified by GABA labelling. Basal AGB labelling within depolarizing bipolar cells was also noted. This basal bipolar cell AGB labelling was not modulated by NMDA and was completely abolished by the use of L-2-amino-4-phosphono-butyric acid, which is known to hyperpolarize retinal depolarizing bipolar cells. AGB is therefore not only useful as a probe of ligand-gated drive, but can also identify neurons that have constitutively open cationic channels. In combination, the NMDA receptor subunit distribution pattern and the AGB gating experiments strongly suggests that this ionotropic glutamate receptor is functional in the cone-driven pathway of the inner retina.
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页码:587 / 597
页数:11
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