Induction of variability through in vivo mutagenesis in chrysanthemum (Chrysanthemum morifolium Ramat.) var. Jaya

The present investigation was carried out in 2013-2014 on Induction of variability through in vivo mutagenesis in chrysanthemum (Chrysanthemum morifolium Ramat.) var. Jaya. The experiment comprised application of two chemical mutagens, viz., EMS and DES (0.02, 0.03 and 0.04%), one physical mutagen, i.e. Gamma rays (5, 10, 15, 20 and 25 Gray) including control and replicated thrice in Randomized Block Design. Lower doses of chemical and physical mutagens, viz., 0.02 per cent EMS and DES and 5, 10 and 15 Gy gamma rays showed positive effect on growth related attributes as compared to higher doses. Lower doses of gamma rays (5, 10 and 15 Gy) altered the flowering behaviour from late to early. Distinct flower mutants were isolated with desirable colours with different gamma-ray doses, viz., light pink (10 Gy), yellow (20 Gy) and brick red with yellowish disc florets (20 Gy). Gamma rays created more variation as compared to EMS and DES in chrysanthemum.