Construction of a derivative of Agrobacterium tumefaciens C58 that does not mutate to tetracycline resistance

被引：102

作者：

Luo, ZQ

Clemente, TE

Farrand, SK ^{[1
]}

机构：

[1] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA

[2] Univ Illinois, Dept Microbiol, Urbana, IL 61801 USA

[3] Univ Nebraska, Ctr Biotechnol, Lincoln, NE 68588 USA

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 2001年 / 14卷 / 01期

关键词：

genetic engineering;

D O I：

10.1094/MPMI.2001.14.1.98

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Agrobacterium tumefaciens C58 mutates to tetracycline resistance at high frequency, complicating the use of many broad-host-range cloning and binary vectors that code for resistance to this antibiotic as the selection marker. Such mutations are associated with a resistant gene unit, tet(C58), that is present in the genome of this strain. By deleting the tet(C58) locus, we constructed NTL4, a derivative of C58 that no longer mutates to tetracycline resistance. The deletion had no detectable effect on genetic or physiological traits of NTL4 or on the ability of this strain to transform plants.

引用

页码：98 / 103

页数：6

共 50 条

[1] Cloning and characterization of a tetracycline resistance determinant present in Agrobacterium tumefaciens C58
Luo, ZQ
Farrand, SK
[J]. JOURNAL OF BACTERIOLOGY, 1999, 181 (02) : 618 - 626
[2] CONSTRUCTION OF AN AGROBACTERIUM-TUMEFACIENS C58 RECA MUTANT
FARRAND, SK
OMORCHOE, SP
MCCUTCHAN, J
[J]. JOURNAL OF BACTERIOLOGY, 1989, 171 (10) : 5314 - 5321
[3] Informational structure of Agrobacterium tumefaciens C58 genome
Liu, Zhihua
Sun, Xiao
[J]. LIFE SYSTEM MODELING AND SIMULATION, PROCEEDINGS, 2007, 4689 : 153 - +
[4] The genome of the natural genetic engineer Agrobacterium tumefaciens C58
Wood, DW
Setubal, JC
Kaul, R
Monks, DE
Kitajima, JP
Okura, VK
Zhou, Y
Chen, L
Wood, GE
Almeida, NF
Woo, L
Chen, YC
Paulsen, IT
Eisen, JA
Karp, PD
Bovee, D
Chapman, P
Clendenning, J
Deatherage, G
Gillet, W
Grant, C
Kutyavin, T
Levy, R
Li, MJ
McClelland, E
Palmieri, A
Raymond, C
Rouse, G
Saenphimmachak, C
Wu, ZN
Romero, P
Gordon, D
Zhang, SP
Yoo, HY
Tao, YM
Biddle, P
Jung, M
Krespan, W
Perry, M
Gordon-Kamm, B
Liao, L
Kim, S
Hendrick, C
Zhao, ZY
Dolan, M
Chumley, F
Tingey, SV
Tomb, JF
Gordon, MP
Olson, MV
[J]. SCIENCE, 2001, 294 (5550) : 2317 - 2323
[5] MOLECULAR ANALYSIS OF THE RECA GENE OF AGROBACTERIUM-TUMEFACIENS C58
WARDHAN, H
MCPHERSON, MJ
HARRIS, CA
SHARMA, E
SASTRY, GRK
[J]. GENE, 1992, 121 (01) : 133 - 136
[6] CLONING AND CHARACTERIZATION OF THE RECA GENE OF AGROBACTERIUM-TUMEFACIENS C58
MILES, CA
MOUNTAIN, A
SASTRY, GRK
[J]. MOLECULAR & GENERAL GENETICS, 1986, 204 (01): : 161 - 165
[7] CONSTRUCTION AND USE OF AGROBACTERIUM-TUMEFACIENS BINARY VECTORS WITH A-TUMEFACIENS C58 T-DNA GENES
VLASAK, J
ONDREJ, M
[J]. FOLIA MICROBIOLOGICA, 1992, 37 (03) : 227 - 230
[8] Overexpression and characterization of hydantoin racemase from Agrobacterium tumefaciens C58
Heras-Vázquez, FJL
Martínez-Rodríguez, S
Mingorance-Cazorla, L
Clemente-Jiménez, JM
Rodríguez-Vico, F
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Joyner, Jessica
Muth, Theodore R.
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[10] Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58
Goodner, B
Hinkle, G
Gattung, S
Miller, N
Blanchard, M
Qurollo, B
Goldman, BS
Cao, YW
Askenazi, M
Halling, C
Mullin, L
Houmiel, K
Gordon, J
Vaudin, M
Iartchouk, O
Epp, A
Liu, F
Wollam, C
Allinger, M
Doughty, D
Scott, C
Lappas, C
Markelz, B
Flanagan, C
Crowell, C
Gurson, J
Lomo, C
Sear, C
Strub, G
Cielo, C
Slater, S
[J]. SCIENCE, 2001, 294 (5550) : 2323 - 2328

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