CONSTRUCTION AND USE OF AGROBACTERIUM-TUMEFACIENS BINARY VECTORS WITH A-TUMEFACIENS C58 T-DNA GENES

被引：10

作者：

VLASAK, J

ONDREJ, M

机构：

[1] Institute of Plant Molecular Biology, Czechoslovak Academy of Sciences, České Budějovice

来源：

FOLIA MICROBIOLOGICA | 1992年 / 37卷 / 03期

关键词：

D O I：

10.1007/BF02933153

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Five plant morphoregulatory genes were isolated from the Agrobacterium tumefaciens Ti plasmid and binary plasmid vectors for plant transformation with these genes were constructed. All vectors have a similar structure with T-DNA borders, RK2 origin of replication and chimeric kanamycin resistance gene for the selection of transformed plant tissues. Over twenty vectors with single and combined morphoregulatory genes were constructed and their effects after tobacco tissue transformation studied.

引用

页码：227 / 230

页数：4

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[1] THE NOPALINE C58 T-DNA REGION IS TRANSCRIBED IN AGROBACTERIUM-TUMEFACIENS
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