Non-invasive live-cell measurement of chancres in macrophage NAD(P)H by two-photon microscopy

被引：10
|
作者：
Kable, EPW
Kiemer, AK
机构：
[1] Univ Sydney, Australian Key Ctr Microscopy & Microanal, Electron Microscope Unit, Sydney, NSW 2006, Australia
[2] Univ Munich, Ctr Drug Res, Dept Pharm, D-81377 Munich, Germany
来源：
IMMUNOLOGY LETTERS | 2005年 / 96卷 / 01期
关键词：
NADPH oxidase; macrophages; reactive oxygen species; autofluorescence;
D O I：
10.1016/j.imlet.2003.12.013
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Two-photon microscopy allows determination of UV-excitable fluorophores using long-wavelength light. We aimed to determine NAD(P)H(P)H autofluorescence as a measure for macrophage NADPH-oxidase activation. RAW264.7 macrophages were grown on glass coverslips and kept in HBSS for microscopic investigation. Cells were excited with 710nm light and NAD(P)H autofluorescence was detected. Glucose as well as NaCN evoked an increase of NAD(P)H autofluorescence. Activators of NADPH oxidase lead to significantly decreased NAD(P)H autofluorescence. Therefore, this work shows the suitability of two-photon microscopy as a non-invasive method determining changes in phagocyte NAD(P)H upon activation. (C) 2004 Elsevier B.V. All rights reserved.
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页码：33 / 38
页数：6
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