In vivo optical bioluminescence imaging of collagen-supported cardiac cell grafts

被引:24
|
作者
Kutschka, Ingo
Chen, Ian Y.
Kofidis, Theo
von Degenfeld, Georges
Sheikh, Ahmad Y.
Hendry, Stephen L.
Hoyt, Grant
Pearl, Jeremy
Blau, Helen M.
Gambhir, Sanjiv S.
Robbins, Robert C.
机构
[1] Stanford Univ, Sch Med, Dept Cardiothorac Surg, Stanford, CA 94305 USA
[2] Stanford Univ, Sch Med, Mol Imaging Program, Bio X Program,Dept Radiol, Stanford, CA 94305 USA
[3] Stanford Univ, Sch Med, Dept Bioengn, Stanford, CA 94305 USA
[4] Stanford Univ, Sch Med, Baxter Lab Genet Pharmacol, Dept Microbiol & Immunol, Stanford, CA 94305 USA
来源
JOURNAL OF HEART AND LUNG TRANSPLANTATION | 2007年 / 26卷 / 03期
关键词
D O I
10.1016/j.healun.2006.11.604
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background: Histology-based survival assessment of cell grafts does not allow for in vivo follow-up. in this study we introduce two new experimental models for longitudinal in vivo survival studies of cardiac cell grafts using optical bioluminescence imaging. Methods: H9c2 cardiomyoblasts expressing both firefly luciferase (fluc) and green fluorescent protein (GFP) reporter genes were implanted into Lewis rats. In Model 1, H9c2-fluc-IRES-GFP cells (0.5 X 10(6)) were implanted into a cryoinjured abdominal wall muscle. Cells were injected using either liquid collagen (Matrigel [MG]) or phosphate-buffered saline (PBS) suspension. Cell survival was evaluated in vivo using bioluminescence imaging on days 1, 5 and 10 post-operatively. In model 2, rats underwent ligation of the left anterior descending (LAD) artery. The donor hearts were harvested, and the infarcted region was restored ex situ using 1 X 10(6) H9c2-fluc-IRES-GFP cells seeded in collagen matrix (Gelfoam [GF]) or suspended in PBS (n = 8/group). Hearts were then transplanted into the abdomen of syngeneic recipients. Optical bioluminescence imaging was performed on Days 1, 5, 8 and 14 post-operatively. After 4 weeks, immunohistologic studies were performed. Results: For model 1, at day 5, bioluminescence signals were markedly higher for the H9c2/MG group (449 +/-- 129 photons/second X 10(3)) compared with the H9c2/PBS group (137 +/- 82 photons/second X 10(3)) (p < 0.05). For model 2, bioluminescence signals were significantly (p < 0.04) higher in the H9c2/GF group compared with plain cell injection on days 5 (534 +/- 115 vs 219 +/- 34) and 8 (274 +/- 34 vs 180 +/- 23). Data were in accordance with GFP immunohistology. Conclusions: Optical biolumiescence is a powerful method for assessment of cardiac cell graft survival in vivo. Collagen matrices support early survival of cardiomyoblasts after transplantation into injured musculature.
引用
收藏
页码:273 / 280
页数:8
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