Stepwise solid-phase synthesis of peptide-oligonucleotide phosphorothioate conjugates employing Fmoc peptide chemistry

被引：27
|
作者：
Antopolsky, M [1 ]
Azhayev, A [1 ]
机构：
[1] Univ Kuopio, Dept Pharmaceut Chem, FIN-70211 Kuopio, Finland
来源：
TETRAHEDRON LETTERS | 2000年 / 41卷 / 47期
关键词：
D O I：
10.1016/S0040-4039(00)01627-0
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
A straightforward stepwise method for the preparation of peptide-oligonucleotide phosphorothioate conjugates, was developed, based on the highly efficient Fmoc peptide solid phase synthesis, followed by oligonucleotide phosphorothiate chain assembly. The three conjugates synthesized contained 15- or 17-mer oligonucleotide phosphorothioates and 10- or 16-mer peptides, incorporating two or three arginine residues. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：9113 / 9117
页数：5
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