Quantitation of serum angiopoietin-like proteins 3 and 4 in a Finnish population sample

被引:94
|
作者
Robciuc, Marius R. [1 ]
Tahvanainen, Esa [2 ]
Jauhiainen, Matti [1 ]
Ehnholm, Christian [1 ]
机构
[1] Biomedicum, Publ Hlth Genom Res Unit, Natl Inst Hlth & Welf, Dept Chron Dis Prevent, Helsinki, Finland
[2] Univ Helsinki, Dept Med Genet, Helsinki, Finland
关键词
ELISA; lipoproteins; triacylglycerols; high density lipoproteins; body weight; body mass index; serum lipids; post-heparin plasma; HIGH-DENSITY LIPOPROTEIN; INDUCED ADIPOSE FACTOR; FATTY-ACIDS; LIPASE; METABOLISM; HDL; HYPERLIPIDEMIA; TRIGLYCERIDE; ANGPTL3; HUMANS;
D O I
10.1194/jlr.M002618
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have developed and validated quantitative ELISAs for human angiopoietin-like (ANGPTL) 3 and 4 and correlated their serum levels with parameters of lipid and carbohydrate metabolism. For this study, we used a random subsample of the Health 2000 Health Examination Survey consisting of 125 men and 125 women, aged 30-94 years. The anthropometric and biochemical parameters of subjects were characterized in detail. ANGPTL 3 and 4 levels were determined using the developed ELISAs. The intra- and inter-assay coefficients of variation for the assays were less than 15%. The average serum concentration of ANGPTL3 was 368 +/- 168 ng/ml (mean +/- SD) and for ANGPTL4 it was 18 +/- 23 ng/ml (mean +/- SD). ANGPTL4 serum levels displayed high variability between individuals ranging from 2 to 158 ng/ml. In post-heparin plasma, both ANGPTL 3 and 4 were increased. Low levels of ANGPTL3 were associated with decreased HDL-cholesterol and increased triglyceride levels. ANGPTL4 levels were positively correlated with FFAs (P = 0.044) and waist-hip ratio (P = 0.016). The developed ELISAs will be important tools to clarify the role of ANGPTL 3 and 4 in human energy metabolism and partitioning of triglycerides between sites of storage (adipose tissue) and oxidation (skeletal and cardiac muscle).-Robciuc, M. R., E. Tahvanainen, M. Jauhiainen, and C. Ehnholm. Quantitation of serum angiopoietin-like proteins 3 and 4 in a Finnish population sample. J. Lipid Res. 2010. 51: 824-831.
引用
收藏
页码:824 / 831
页数:8
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