Determination of Protein in Human Serum by Resonance Light Scattering Method Using Methylene Blue

被引：0
|
作者：
Liu, Benzhi [1 ]
Lu, Liyuan [1 ]
Wang, Min [1 ]
Yu, Zhang [1 ]
Huang, Yabei [1 ]
Zi, Yanqin [1 ]
机构：
[1] Huaibei Coal Normal Coll, Dept Chem, Huaibei 235000, Peoples R China
来源：
CHEMIA ANALITYCZNA | 2009年 / 54卷 / 05期
关键词：
Methylene blue; Sodium dodecyl sulfate; Human serum albumin; Resonance light scattering; UV-VIS spectrophotometry; ENHANCEMENT;
D O I：
暂无
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Binding reaction of methylene blue (MB) with human serum albumin (HSA) in the presence of sodium dodecyl sulfate (SDS) was studied. It was found that at pH = 2.87 positively charged MB bound firstly to HSA via hydro.-en and hydrophobic bonds to form MB-HSA aggregate. The aggregate contained many positive charges and attracted negatively charged SDS to form macromolecules, which induced a great enhancement of RLS intensity. RLS intensity was directly proportional to the concentration of HSA. This phenomenon was utilised in a new method for quantitative determination of HSA protein.
引用
收藏
页码：975 / 987
页数：13
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