Antibodies to long-chain acyl-CoAs - A new tool for lipid biochemistry

被引：8

作者：

Maneta-Peyret, L ^{[1
]}

Sturbois-Balcerzak, B ^{[1
]}

Cassagne, C ^{[1
]}

Moreau, P ^{[1
]}

机构：

[1] Univ Bordeaux 2, CNRS, UMR 5544, F-33076 Bordeaux, France

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM | 1998年 / 1389卷 / 01期

关键词：

acyl-CoA; ELISA; antibody; amphiphilic molecule;

D O I：

10.1016/S0005-2760(97)00146-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Antibodies directed against long chain acyl-CoAs (having 16 and 18 carbon atoms) have been prepared and are reported for the first time. A modified ELISA procedure adapted to these amphiphilic molecules has been developed: it is a rapid, simple and sensitive test permitting to detect as little as 3 pmol of acyl-CoA. These antibodies represent a new tool for studying long-chain acyl-CoAs. Their use in an immunochemical approach for the study of protein-acyl-CoA interactions is presented. (C) 1998 Elsevier Science B.V.

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页码：50 / 56

页数：7

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