Functionalization of Nitrogen-Doped Carbon Nanotubes by 1-Pyrenebutyric Acid and Its Application for Biosensing

被引：5
|
作者：
Xu, Xuan [1 ]
Yu, Jiachao [1 ]
Qian, Jing [1 ]
Cui, Dongmei [1 ]
Liu, Songqin [1 ]
机构：
[1] Southeast Univ, Sch Chem & Chem Engn, Nanjing 210096, Jiangsu, Peoples R China
来源：
IEEE SENSORS JOURNAL | 2014年 / 14卷 / 07期
基金：
中国国家自然科学基金;
关键词：
Glucose biosensing; glucose oxidase; nitrogen-doped carbon nanotubes; 1-pyrenebutyric acid; DIRECT ELECTRON-TRANSFER; GLUCOSE-OXIDASE; DIRECT ELECTROCHEMISTRY; GRAPHENE; LACCASE; FILM;
D O I：
10.1109/JSEN.2014.2309974
中图分类号：
TM [电工技术]; TN [电子技术、通信技术];
学科分类号：
0808 ; 0809 ;
摘要：
The homogeneous nanocomposites of 1-pyrene butyric acid modified nitrogen-doped carbon nanotubes (PBA/NCNTs) are synthesized for the immobilization of enzymes and glucose biosensing. Through the aqueous carbodiimide coupling chemistry, glucose oxidase (GOD) is covalently immobilized on the surface of the as-prepared PBA/NCNTs with high enzyme loading (1.986 nmol.cm(-2)). Fast direct electron transfer between GOD and the electrode is observed with the electron transfer rate constant (ks) of 2.43 s(-1). Besides, the GOD/PBA/NCNTs-based biosensor exhibits a linear response to glucose concentrations ranging from 2 mu M to 0.2 mM with the detection limit of 1.33 mu M and the high sensitivity of 179.76 mu A.mM(-1) . cm(-2). Furthermore, this biosensor is also applied to detect glucose in the human serum samples with high accuracy. These results demonstrate that the PBA/NCNTs nanocomposites can be a promising platform for immobilizing biomolecules and biosensing application.
引用
收藏
页码：2341 / 2346
页数：6
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