Determination of trans-resveratrol in wine by micro-HPLC with fluorescence detection

被引：17
|
作者：
Lopez, Ricardo
Dugo, Paola
Mondello, Luigi
机构：
[1] Univ Zaragoza, Dept Analyt Chem, Fac Sci, Zaragoza 50009, Spain
[2] Univ Messina, Dipartimento Sci Alimenti & Ambiente, Fac Sci, Messina, Italy
[3] Univ Messina, Dipartimento Farm Chim, Fac Farm, Messina, Italy
来源：
JOURNAL OF SEPARATION SCIENCE | 2007年 / 30卷 / 05期
关键词：
fluorescence; micro-HPLC; trans-resveratrol; wine;
D O I：
10.1002/jssc.200600300
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A new method for the quantitative determination of trans-resveratrol in wine has been optimized and validated. The method is based on the direct injection of 500 nL of wine in an HPLC system fitted with an RP microcolumn of 10 cm x 0.32 mm and spectrofluorometric detection. The linear dynamic range of the method covers the normal range of occurrence of the analyte in wines and extends for two orders of magnitude with r(2) = 0.9994. Twenty-three wines have been analyzed with the proposed method, finding concentrations in the range reported by other authors.
引用
收藏
页码：669 / 672
页数：4
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