Head kidney neutrophils of carp (Cyprinus carpio L.) are functionally modulated by the haemoflagellate Trypanoplasma borreli

被引:17
|
作者
Scharsack, JP
Steinhagen, D
Kleczka, C
Schmidt, JO
Körting, W
Michael, RD
Leibold, W
Schuberth, HJ
机构
[1] Hannover Sch Vet Med, Fish Dis Res Unit, D-30545 Hannover, Germany
[2] Hannover Sch Vet Med, Immunol Unit, D-30545 Hannover, Germany
[3] Amer Coll, Dept Zool, Madurai 625002, Tamil Nadu, India
关键词
carp; granulocytes; phagocytosis; nitric oxide; reactive oxygen species; Trypanoplasma borreli;
D O I
10.1006/fsim.2002.0447
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
In the present work responses of carp (Cyprinus carpio) head kidney-derived neutrophils to the blood parasite T. borreli, and the consequences of these responses for parasite survival and other host response mechanisms,. were studied. In co-cultures of head kidney leucocytes (HKL) with viable and lysed T. borreli a prominent shape change of neutrophilic granulocytes towards increased size and complexity was observed. In addition, the longevity of neutrophils in vitro was prolonged in the presence of T. borreli antigens. In these cultures, neutrophils also exhibited an increased phagocytosis activity. An up regulation of the production of nitric oxide (NO) and reactive oxygen species (ROS) was observed in T. borreli- and mitogen-stimulated HKL cultures. However, addition of live, fluorescence-labelled T. borreli to previously stimulated HKL cultures, revealed neither killing nor phagocytosis of the parasite by activated neutrophils. Moreover, viable T. borreli, when added to HKL cultures of infected carp, reduced their phagocytosis activity and NO production. Supernatants of co-cultures between T. borreli and HKL also contained mediators, which suppressed a mitogen-induced proliferative response of peripheral blood leucocytes (PBL) in vitro. Thus, while T. borreli itself appeared not to be sensitive to responses of activated neutrophils, the flagellates interferes with the production of immunomodulatory signals of these cells, probably resulting in a partial immunosuppression, which may favour the parasite development in vivo. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:389 / 403
页数:15
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