Amperometric glutamate biosensor based on poly(o-phenylenediamine) film electrogenerated onto modified carbon paste electrodes

被引：52
|
作者：
Alvarez-Crespo, SL [1 ]
Lobo-Castanon, MJ [1 ]
Miranda-Ordieres, AJ [1 ]
Tunon-Blanco, P [1 ]
机构：
[1] Univ Oviedo, Dept Quim Fis & Analit, Oviedo 33006, Asturias, Spain
来源：
BIOSENSORS & BIOELECTRONICS | 1997年 / 12卷 / 08期
关键词：
glutamate; poly(O-phenylenediamine) amperometric biosensor; carbon paste;
D O I：
10.1016/S0956-5663(97)00041-9
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
A glutamate biosensor was developed by electropolymerizing o-phenylenediamine on a dehydrogenase-NAD(+) modified carbon paste electrode. The amperometric response to glutamate was obtained by means of the electrocatalytic oxidation of the enzymatically produced NADH, at an applied potential close to OV (Ag/AgCl). After optimizing carbon paste composition, polymer thickness and operating variables, a linear amperometric response to glutamate was obtained within the concentration range 5.0 x 10(-6)-7.8 x 10(-5) M with a detection limit of 3.8 x 10(-6) M. The biosensor was applied to the determination of glutamate in chicken bouillon cubes. Good accuracy was found with respect to a reference enzymatic spectrophotometric method. (C) 1997 Elsevier Science Limited.
引用
收藏
页码：739 / 747
页数：9
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