A Synthetic Biology Perspective on the Bioengineering Tools for an Industrial Microalga: Euglena gracilis

被引:8
|
作者
Chen, Zhenfan [1 ,2 ,3 ]
Zhu, Jiayi [2 ]
Du, Ming [2 ]
Chen, Zixi [2 ]
Liu, Qiong [2 ,4 ]
Zhu, Hui [1 ]
Lei, Anping [2 ]
Wang, Jiangxin [2 ]
机构
[1] Hanshan Normal Univ Coll Food Engn & Biotechnol Ch, Chaozhou, Peoples R China
[2] Shenzhen Univ, Coll Life Sci & Oceanog, Shenzhen Engn Lab Marine Algal Biotechnol Guangdon, Shenzhen Key Lab Marine Bioresource Ecoenvironm S, Shenzhen, Peoples R China
[3] Shenzhen Univ, Coll Optoelect Engn, Key Lab Optoelect Devices Syst, Minist Educ Guangdong Prov, Shenzhen, Peoples R China
[4] Hong Kong Inst Brain Sci Shenzhen Peoples R China, Shenzhen, Peoples R China
基金
中国国家自然科学基金;
关键词
Euglena gracilis; genetic transformation; biotechnology; CRISPR; RNAi; WAX ESTER FERMENTATION; SIGNAL-TRANSDUCTION; PHYSIOLOGICAL-ROLE; NUCLEAR GENOME; TRANSFORMATION; ELECTROPORATION; MICROINJECTION; IDENTIFICATION; CHLAMYDOMONAS; EXPRESSION;
D O I
10.3389/fbioe.2022.882391
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Euglena is a genus of single-celled eukaryotes that show both plant- and animal-like characteristics. Euglena gracilis, a model species, is of great academic interest for studying endosymbiosis and chloroplast development. As an industrial species, E. gracilis is also of primary biotechnological and economic importance as high value-added food, medicine, and cosmetic and high-quality feedstock for jet-fuel production because of its cells containing many high-value products, such as vitamins, amino acids, pigments, unsaturated fatty acids, and carbohydrate paramylon, as metabolites. For more than half a century, E. gracilis has been used as an industrial biotechnology platform for fundamental biology research, mainly exploring relevant physiological and biochemical method studies. Although many researchers focused on genetic engineering tools for E. gracilis in recent years, little progress has been achieved because of the lack of high-quality genome information and efficient techniques for genetic operation. This article reviewed the progress of the genetic transformation of E. gracilis, including methods for the delivery of exogenous materials and other advanced biotechnological tools for E. gracilis, such as CRISPR and RNA interference. We hope to provide a reference to improve the research in functional genomics and synthetic biology of Euglena.
引用
收藏
页数:7
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