Cryopreservation of zygotic embryos of the Brazilian Green Dwarf coconut

被引：0

作者：

Ledo, Ana da Silva ^{[1
]}

Jenderek, Maria ^{[2
]}

Skogerboe, Dianne ^{[2
]}

Staats, Elise ^{[2
]}

Machado, Caroline de Araujo ^{[3
]}

Resende de Oliveira, Leila Albuquerque ^{[3
]}

机构：

[1] Embrapa Tabuleiros Costeiros, Ave Beira Mar 3-250, BR-49025040 Aracaju, SE, Brazil

[2] ARS, USDA, 1111 South Mason St, Ft Collins, CO 80526 USA

[3] Univ Fed Sergipe, Campus Sao Cristovao, BR-49100000 Sao Cristovao, SE, Brazil

来源：

PESQUISA AGROPECUARIA BRASILEIRA | 2018年 / 53卷 / 04期

基金：

美国农业部;

关键词：

Cocos nucifera; ex situ conservation; PVS3; NUCIFERA L; VITRIFICATION; REGENERATION; VIABILITY;

D O I：

10.1590/S0100-204X2018000400013

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The objective of this work was to evaluate rewarming procedures and recovery media for Brazilian Green Dwarf coconut (Cocos nucifera) embryos cryopreserved by vitrification. The rewarming procedures evaluated were: T1, water bath at 38 +/- 2 degrees C; and T2, unloading solution consisting of Y3 medium + 1.2 mol L-1 sucrose. The recovery media assessed were: M1, Y3 medium + 45 g L-1 sucrose + 1 mg L-1 gibberellic acid + 1 g L-1 activated charcoal + 2.2 g L-1 Gelrite; and M2, Y3 medium + 60 g L-1 sucrose + 2.2 g L-1 Gelrite. Rewarming procedures showed no significant effect, and the M1 media induced 72.5% regeneration.

引用

页码：514 / 517

页数：4

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