Cryopreservation of zygotic embryos of the Brazilian Green Dwarf coconut

被引：0
|
作者：
Ledo, Ana da Silva [1 ]
Jenderek, Maria [2 ]
Skogerboe, Dianne [2 ]
Staats, Elise [2 ]
Machado, Caroline de Araujo [3 ]
Resende de Oliveira, Leila Albuquerque [3 ]
机构：
[1] Embrapa Tabuleiros Costeiros, Ave Beira Mar 3-250, BR-49025040 Aracaju, SE, Brazil
[2] ARS, USDA, 1111 South Mason St, Ft Collins, CO 80526 USA
[3] Univ Fed Sergipe, Campus Sao Cristovao, BR-49100000 Sao Cristovao, SE, Brazil
来源：
PESQUISA AGROPECUARIA BRASILEIRA | 2018年 / 53卷 / 04期
基金：
美国农业部;
关键词：
Cocos nucifera; ex situ conservation; PVS3; NUCIFERA L; VITRIFICATION; REGENERATION; VIABILITY;
D O I：
10.1590/S0100-204X2018000400013
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
The objective of this work was to evaluate rewarming procedures and recovery media for Brazilian Green Dwarf coconut (Cocos nucifera) embryos cryopreserved by vitrification. The rewarming procedures evaluated were: T1, water bath at 38 +/- 2 degrees C; and T2, unloading solution consisting of Y3 medium + 1.2 mol L-1 sucrose. The recovery media assessed were: M1, Y3 medium + 45 g L-1 sucrose + 1 mg L-1 gibberellic acid + 1 g L-1 activated charcoal + 2.2 g L-1 Gelrite; and M2, Y3 medium + 60 g L-1 sucrose + 2.2 g L-1 Gelrite. Rewarming procedures showed no significant effect, and the M1 media induced 72.5% regeneration.
引用
收藏
页码：514 / 517
页数：4
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