SOYBEAN (GLYCINE MAX L.) GERMPLASM SCREENING AND GEOGRAPHICAL DETERMINATION BASED ON TARGETED ISOFLAVONE METABOLOMICS

被引:3
|
作者
Iqbal, N. [1 ]
Zhang, Q. [1 ]
Wu, H. [1 ]
Yang, C. [1 ]
Deng, J. [1 ]
Qin, W. [1 ]
Zhang, J. [1 ]
Yang, W. [1 ]
Liu, J. [1 ,2 ]
机构
[1] Minist Agr, Key Lab Crop Ecophysiol & Farming Syst Southwest, Chengdu 611130, Sichuan, Peoples R China
[2] Sichuan Agr Univ, Inst Ecol Agr, Chengdu 611130, Sichuan, Peoples R China
来源
APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH | 2018年 / 16卷 / 04期
基金
中国博士后科学基金; 中国国家自然科学基金;
关键词
community size; environment; latitude; multivariate analysis; metabolite; ENVIRONMENT INTERACTION; SEED DEVELOPMENT; GENOTYPE; CHINA; TEMPERATURE; METABOLISM; CULTIVARS; VARIETY; REVEALS; GROWTH;
D O I
10.15666/aeer/1604_39333953
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
In this study, the combination of high-performance liquid chromatography (HPLC) based isoflavone profiling with multivariate analysis was used to screen specific soybean germplasms from Southwestern China, and simultaneously to investigate the influence of genotype, the geographical environment and the germplasm community size on isoflavone biosynthesis. A total of 144 soybean germplasms were evaluated for 12 isoflavones, which varied markedly due to genotypes and locations. Partial least squares analysis (PLS) results exhibited little variability among the 144 samples of four different locations. Five samples were separated from other genotypes based on the first PLS principal component that had high isoflavones. Daidzin, genistin, malonyldaidzin, malonylgenistin and acetylgenistin were the discriminating metabolites. Later the bidirectional orthogonal projection to latent structures-discriminant analysis (O2PLS-DA) was applied for geographical determination of five specific germplasms samples. Geographical environment influenced mainly acetylgenistin, acetyldaidzin, glycitein and malonylglycitin. Our results suggest that genetic factors play a vital role in the isoflavones biosynthesis of a major community, whereas the environmental factors can effectively regulate the isoflavones accumulation especially in the evaluation of small groups of soybean germplasms. These results demonstrate the use of the HPLC based metabolite profiling combined with multivariate analysis (PLS and O2PLS-DA) as a tool for the determination of specific germplasm, biomarker metabolite and geographical origin.
引用
收藏
页码:3933 / 3953
页数:21
相关论文
共 50 条
  • [41] Pollination of soybean (Glycine max L. Merril) by honeybees (Apis mellifera L.)
    Chiari, WC
    de Toledo, VDA
    Ruvolo-Takasusuki, MCC
    de Oliveira, AJB
    Sakaguti, ES
    Attencia, VM
    Costa, FM
    Mitsui, MH
    BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY, 2005, 48 (01) : 31 - 36
  • [42] Biochemical Characterization of Esterase from Soybean (Glycine max L.)
    de Barros, Marcio
    Macedo, Gabriela Alves
    FOOD SCIENCE AND BIOTECHNOLOGY, 2011, 20 (05) : 1195 - 1201
  • [43] Priming Fresh and Aged Seed of Soybean (Glycine max L.)
    Miladinov, Z.
    Maksimovic, I
    Tubic, S. Balesevic
    Canak, P.
    Miladinovic, J.
    Djukic, V
    Randjelovic, P.
    LEGUME RESEARCH, 2021, 44 (04) : 452 - 457
  • [44] Recombination hotspots in soybean [Glycine max (L.) Merr.]
    McConaughy, Samantha
    Amundsen, Keenan
    Song, Qijian
    Pantalone, Vince
    Hyten, David
    G3-GENES GENOMES GENETICS, 2023, 13 (06):
  • [45] Viruses infecting soybean (Glycine max L. Merill) in Nigeria
    Time, I.
    Atiri, G. I.
    Kumar, P. Lava
    PHYTOPATHOLOGY, 2010, 100 (06) : S126 - S126
  • [46] Identification of QTLs for branching in soybean (Glycine max (L.) Merrill)
    Sangrea Shim
    Moon Young Kim
    Jungmin Ha
    Yeong-Ho Lee
    Suk-Ha Lee
    Euphytica, 2017, 213
  • [47] Biochemical characterization of esterase from soybean (Glycine max L.)
    Márcio de Barros
    Gabriela Alves Macedo
    Food Science and Biotechnology, 2011, 20 : 1195 - 1201
  • [48] Identification of QTLs for branching in soybean (Glycine max (L.) Merrill)
    Shim, Sangrea
    Kim, Moon Young
    Ha, Jungmin
    Lee, Yeong-Ho
    Lee, Suk-Ha
    EUPHYTICA, 2017, 213 (09)
  • [49] Economics of weed control in soybean (Glycine max (L.) Merrill)
    Basavaraju, H. K.
    Nanjappa, H. V.
    Journal of Oilseeds Research, 13 (01):
  • [50] Comparative Metabolomics Analysis of Seed Composition Accumulation in Soybean (Glycine max L.) Differing in Protein and Oil Content
    Cui, Yifan
    Wang, Zhiyang
    Li, Mingyang
    Li, Xin
    Wang, Sihui
    Liu, Chunyan
    Xin, Dawei
    Qi, Zhaoming
    Chen, Qingshan
    Yang, Mingliang
    Zhao, Ying
    PLANT CELL AND ENVIRONMENT, 2025,