High-affinity monoclonal antibodies for detection of the microbial metabolite, 2-methylisoborneol

被引：4
|
作者：
Plhak, LC
Park, ES
机构：
[1] Louisiana Agr Expt Stn, Dept Food Sci, Baton Rouge, LA 70803 USA
[2] Louisiana State Univ, Baton Rouge, LA 70803 USA
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2003年 / 51卷 / 13期
关键词：
2-methylisoborneol; borneol; monoclonal antibodies; ELISA; immunoassay;
D O I：
10.1021/jf0340207
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
The production of 2-methylisoborneol (MIB) by certain fungi and algae can contribute musty off-flavors to foods and water supplies if uncontrolled. The goal of this research was to develop a nonsensory simple method for the detection of MIB. Anti-MIB monoclonal antibodies were produced by immunizing mice with borneol-conjugated protein and selecting positive clones with an MIB-protein conjugate. An indirect competitive immunoassay developed using this antibody had a detection limit of 0.6 mug L-1 and an 150 value of 5 mug L-1. Detection was relatively specific for MIB and showed 20% cross-reactivity with borneol or isoborneol and 4-5% cross-reactivity with camphor. No cross-reactivity to geosmin was observed.
引用
收藏
页码：3731 / 3736
页数：6
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