Classification of oral streptococci by two-dimensional gel electrophoresis with direct activity stain for glycosyltransferases

被引：2
|
作者：
Ando, T
Tsumori, H
Shimamura, A
Sato, Y
Mukasa, H
机构：
[1] Natl Def Med Coll, Dept Oral & Maxillofacial Surg, Tokorozawa, Saitama 3598513, Japan
[2] Natl Def Med Coll, Dept Chem, Tokorozawa, Saitama 3598513, Japan
来源：
ORAL MICROBIOLOGY AND IMMUNOLOGY | 2003年 / 18卷 / 03期
关键词：
oral streptococci; two-dimensional gel electrophoresis; glycosyltransferases;
D O I：
10.1034/j.1399-302X.2003.00062.x
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
Thirty eight strains of oral streptococci were divided into six types by two-dimensional gel electrophoresis (2-DE) followed by glycosyltransferase (GLT) activity stain: Type 1, Streptococcus mutans; Type 2, Streptococcus rattus; Type 3, Streptococcus sobrinus and Streptococcus downei; Type 4, Streptococcus cricetus; Type 5, Streptococcus salivarius; and Type 6, Streptococcus sanguis , Streptococcus oralis and Streptococcus gordonii . In Types 1, 2 and 5, two glucosyltransferases synthesizing water-insoluble (GTF-I) and water-soluble glucans (GTF-S) and a fructosyltransferase (FTF) were detected. In Types 3 and 4, GTF-I and two GTF-Ss were detected. Only one GTF-S was detected in Type 6. The 2-DE patterns for these six types were clearly distinguished from each other based on the kind, number and location of GLTs in gel.
引用
收藏
页码：171 / 175
页数：5
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