Isolation and characterization of Acinetobacter sp ES-1 excreting a lipase with high enantioselectivity for (S)-ketoprofen ethyl ester

被引：7

作者：

Lee, KW ^{[1
]}

Shin, GS ^{[1
]}

Bae, HA ^{[1
]}

Shin, HD ^{[1
]}

Lee, YH ^{[1
]}

机构：

[1] Kyungpook Natl Univ, Coll Nat Sci, Dept Genet Engn, Taegu 702701, South Korea

来源：

BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 21期

关键词：

Acinetobacter; enantioselective; lipase; (R; S)-ketoprofen ethyl ester; (S)-ketoprofen;

D O I：

10.1007/s10529-004-3512-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new Acinetobacter sp. ES-1, grown on triolein, tryptone and Triton X-100, excreted a lipase that hydrolyzed 10 mM (R,S)-ketoprofen ethyl ester into (S)-ketoprofen. The crude lipase had an activity of 10 U ml(-1) and, at 30degreesC and pH 7 over 48 h, gave a conversion yield of 35% with an enantiomeric excess for the product 96%.

引用

页码：1639 / 1642

页数：4

共 11 条

[1] Isolation and characterization of Acinetobacter sp. ES-1 excreting a lipase with high enantioselectivity for (S)-ketoprofen ethyl ester
Kwang-Woo Lee
Gab-Sang shin
Hyun-Ae Bae
Hyun-Dong shin
Yong Hyun lee
[J]. Biotechnology Letters, 2004, 26 : 1639 - 1642
[2] Purification and catalytic properties of novel enantioselective lipase from Acinetobacter sp ES-1 for hydrolysis of (S)-ketoprofen ethyl ester
Lee, KW
Bae, HA
Shin, GS
Lee, YH
[J]. ENZYME AND MICROBIAL TECHNOLOGY, 2006, 38 (3-4) : 443 - 448
[3] Enhanced enantioselectivity of immobilized Candida antarctica lipase for hydrolysis of ketoprofen ethyl ester at pH 1
Jin Chuan Wu
Philip Ho
Tee Yuan Poh
Yvonne Chow
MMR Talukder
Won Jae Choi
[J]. Korean Journal of Chemical Engineering, 2007, 24 : 648 - 650
[4] Enhanced enantioselectivity of immobilized Candida antarctica lipase for hydrolysis of ketoprofen ethyl ester at pH 1
Wu, Jin Chuan
Ho, Philip
Poh, Tee Yuan
Chow, Yvonne
Talukder, M. M. R.
Choi, Won Jae
[J]. KOREAN JOURNAL OF CHEMICAL ENGINEERING, 2007, 24 (04) : 648 - 650
[5] Crystal structure and characterization of esterase Est25 mutants reveal improved enantioselectivity toward (S)-ketoprofen ethyl ester
Jinyeong Kim
Seung-hyeon Seok
Eunsoo Hong
Tae Hyeon Yoo
Min-duk Seo
Yeonwoo Ryu
[J]. Applied Microbiology and Biotechnology, 2017, 101 : 2333 - 2342
[6] Crystal structure and characterization of esterase Est25 mutants reveal improved enantioselectivity toward (S)-ketoprofen ethyl ester
Kim, Jinyeong
Seok, Seung-hyeon
Hong, Eunsoo
Yoo, Tae Hyeon
Seo, Min-duk
Ryu, Yeonwoo
[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2017, 101 (06) : 2333 - 2342
[7] Construction and characterization of a recombinant esterase with high activity and enantio selectivity to (S)-ketoprofen ethyl ester
Choi, GS
Kim, JY
Kim, JH
Ryu, YW
Kim, GJ
[J]. PROTEIN EXPRESSION AND PURIFICATION, 2003, 29 (01) : 85 - 93
[8] Improved enantioselectivity of thermostable esterase from Archaeoglobus fulgidus toward (S)-ketoprofen ethyl ester by directed evolution and characterization of mutant esterases
Jinyeong Kim
Seungbum Kim
Sangyoung Yoon
Eunsoo Hong
Yeonwoo Ryu
[J]. Applied Microbiology and Biotechnology, 2015, 99 : 6293 - 6301
[9] Improved enantioselectivity of thermostable esterase from Archaeoglobus fulgidus toward (S)-ketoprofen ethyl ester by directed evolution and characterization of mutant esterases
Kim, Jinyeong
Kim, Seungbum
Yoon, Sangyoung
Hong, Eunsoo
Ryu, Yeonwoo
[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2015, 99 (15) : 6293 - 6301
[10] Screening, production and properties of a stereospecific esterase from Pseudomonas sp S34 with high selectivity to (S)-ketoprofen ethyl ester
Kim, GJ
Choi, GS
Kim, JY
Lee, JB
Jo, DH
Ryu, YW
[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2002, 17 (01) : 29 - 38

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