Enhanced enantioselectivity of immobilized Candida antarctica lipase for hydrolysis of ketoprofen ethyl ester at pH 1

被引：9

作者：

Wu, Jin Chuan ^{[1
]}

Ho, Philip ^{[1
]}

Poh, Tee Yuan ^{[1
]}

Chow, Yvonne ^{[1
]}

Talukder, M. M. R. ^{[1
]}

Choi, Won Jae ^{[1
]}

机构：

[1] Inst Chem & Engn Sci, Singapore 627833, Singapore

来源：

KOREAN JOURNAL OF CHEMICAL ENGINEERING | 2007年 / 24卷 / 04期

关键词：

Candida antarctica; hydrolysis; ketoprofen; lipase; resolution;

D O I：

10.1007/s11814-007-0018-6

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The immobilized Candida antarctica lipase, a commercially available and one of the most commonly used enzymes, showed significantly improved enantioselectivity (twice) when used in a very acidic environment (pH 1.0) than in the normal pH 7.0 for the hydrolysis of ketoprofen ethyl ester at 45 degrees C. The enzyme was still 60% active at pH 1.0 compared to the activity at pH 7.0 and its stabilities at the two pH values were almost the same. The improved enantioselectivity was ascribed to the conformational change of the enzyme in the very acidic environment.

引用

页码：648 / 650

页数：3

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