Quantitative Analysis of Protein-Lipid Interactions Using Tryptophan Fluorescence

被引：35
|
作者：
Kraft, Catherine A. [1 ]
Garrido, Jose Luis [1 ]
Leiva-Vega, Luis [1 ]
Romero, Guillermo [1 ]
机构：
[1] Univ Pittsburgh, Sch Med, Dept Pharmacol & Chem Biol, Pittsburgh, PA 15261 USA
来源：
SCIENCE SIGNALING | 2009年 / 2卷 / 99期
关键词：
PHOSPHATIDIC-ACID; RAF-1; KINASE; ULTRAVIOLET FLUORESCENCE; MEMBRANE TOPOLOGY; BINDING; INSERTION; TRANSLOCATION; ASSOCIATION; CHOLESTEROL; PEPTIDES;
D O I：
10.1126/scisignal.299pl4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The fluorescent properties of the amino acid tryptophan make it a useful tool for fluorometric assays. Because tryptophan fluorescence is remarkably sensitive to the polarity of the environment, it can be used to determine the affinity of tryptophan-containing peptides for phospholipid vesicles of varying compositions. Here, we describe a method for using tryptophan fluorescence to determine the binding affinities of peptides derived from the proteins Raf-1 and KSR-1 to small unilamellar vesicles containing phosphatidic acid. The method can be extrapolated to measure the binding of other tryptophan-containing peptides or proteins to lipid vesicles.
引用
收藏
页数：7
相关论文
共 50 条
  • [31] Characterization of protein-lipid binding interactions using synthetic probes
    Rowland, Meng M.
    Bostic, Heidi E.
    Gong, Denghuang
    Smith, Matthew D.
    Best, Michael D.
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2010, 239
  • [32] Importance of phospholipid bilayer integrity in the analysis of protein-lipid interactions
    Druecker, Patrick
    Gerke, Volker
    Galla, Hans-Joachim
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2014, 453 (01) : 143 - 147
  • [33] Mechanical properties of lipid bilayers and protein-lipid interactions
    Hianik, T
    ELECTRICITY AND MAGNETISM IN BIOLOGY AND MEDICINE, 1999, : 235 - 238
  • [34] Multidisciplinary analysis of protein-lipid interactions and implications in neurodegenerative disorders
    Collin, F.
    Cerlati, O.
    Couderc, F.
    Lonetti, B.
    Marty, J. -D.
    Mingotaud, A. -F.
    TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2020, 132
  • [35] A protocol for the systematic and quantitative measurement of protein-lipid interactions using the liposome-microarray-based assay
    Saliba, Antoine-Emmanuel
    Vonkova, Ivana
    Deghou, Samy
    Ceschia, Stefano
    Tischer, Christian
    Kugler, Karl G.
    Bork, Peer
    Ellenberg, Jan
    Gavin, Anne-Claude
    NATURE PROTOCOLS, 2016, 11 (06) : 1021 - 1038
  • [36] Protein myristoylation in protein-lipid and protein-protein interactions
    Taniguchi, H
    BIOPHYSICAL CHEMISTRY, 1999, 82 (2-3) : 129 - 137
  • [37] Membrane protein sequestering by ionic protein-lipid interactions
    van den Bogaart, Geert
    Meyenberg, Karsten
    Risselada, H. Jelger
    Amin, Hayder
    Willig, Katrin I.
    Hubrich, Barbara E.
    Dier, Markus
    Hell, Stefan W.
    Grubmueller, Helmut
    Diederichsen, Ulf
    Jahn, Reinhard
    NATURE, 2011, 479 (7374) : 552 - 555
  • [38] PROTEIN-LIPID INTERACTIONS: ROLE OF MEMBRANE PLASTICITY AND LIPID SPECIFICITY ON PERIPHERAL PROTEIN INTERACTIONS
    Murphy, Jesse
    Knutson, Kristofer
    Hinderliter, Anne
    METHODS IN ENZYMOLOGY, VOL 466: BIOTHERMODYNAMICS, PT B, 2009, 466 : 431 - 453
  • [39] PROTEIN-LIPID INTERACTIONS IN CONCENTRATED INFANT FORMULA
    ROWLEY, BO
    RICHARDSON, T
    JOURNAL OF DAIRY SCIENCE, 1985, 68 (12) : 3180 - 3188
  • [40] Role of Protein-Lipid Interactions in Viral Entry
    Ander Nieto-Garai, Jon
    Contreras, Francesc-Xabier
    Arboleya, Aroa
    Lorizate, Maier
    ADVANCED BIOLOGY, 2022, 6 (03):
12345