Immobilization of Enzymes on Functionalized Magnetic Nanoparticles for Efficient Biocatalysis

被引：0

作者：

Wang, W. ^{[1
]}

Li, Z. ^{[2
]}

Wang, D. I. C. ^{[3
]}

机构：

[1] Singapore MIT Alliance, Chem & Pharmaceut Engn Programme, Singapore, Singapore

[2] Natl Univ Singapore, Dept Chem & Biomol Engn, Singapore, Singapore

[3] MIT, Dept Chem Engn, Cambridge, MA 02139 USA

来源：

NANOTECH CONFERENCE & EXPO 2009, VOL 2, TECHNICAL PROCEEDINGS: NANOTECHNOLOGY 2009: LIFE SCIENCES, MEDICINE, DIAGNOSTICS, BIO MATERIALS AND COMPOSITES | 2009年

关键词：

magnetic nanoparticles; superparamagnetism; immobilization of enzymes; BSA; biocatalysis;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this paper, iron oxide magnetic nanoparticles were synthesized by co-precipitation method and were functionalized with aldehyde group. Spherical superparamagnetic nanoparticles with diameter of 100 nm were obtained based on the results of field emission scanning electron microscopy (FESEM) and vibrating sample magnetometer (VSM). These particles were further applied for immobilization of enzymes by covalent binding between aldehyde group on particles and amino group on the enzyme surface. Different pH was applied for optimization of the immobilization conditions. The result showed that pH 4.0 is the best pH for immobilizing BSA on the nanometric support.

引用

页码：337 / +

页数：2

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