Protocol for PTEN Expression by Immunohistochemistry in Formalin-fixed Paraffin-embedded Human Breast Carcinoma

被引：47

作者：

Sakr, Rita A. ^{[2
]}

Barbashina, Violetta ^{[3
]}

Morrogh, Mary ^{[2
]}

Chandarlapaty, Sarat ^{[4
]}

Andrade, Victor P. ^{[2
]}

Arroyo, Crispinita D. ^{[2
]}

Olvera, Narciso ^{[2
]}

King, Tari A. ^{[1
]}

机构：

[1] Mem Sloan Kettering Canc Ctr, Dept Surg, Breast Serv, New York, NY 10065 USA

[2] Mem Sloan Kettering Canc Ctr, Sloan Kettering Inst, New York, NY 10065 USA

[3] Mem Sloan Kettering Canc Ctr, Dept Pathol, New York, NY 10065 USA

[4] Mem Sloan Kettering Canc Ctr, Dept Med Oncol, New York, NY 10065 USA

来源：

APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY | 2010年 / 18卷 / 04期

关键词：

PTEN protein expression; immunohistochemistry; automated protocol; breast cancer; TUMOR-SUPPRESSOR GENE; REDUCED EXPRESSION; PROSTATE-CANCER; TRASTUZUMAB; RESISTANCE; PROTEIN; PATHWAY;

D O I：

10.1097/PAI.0b013e3181d50bd5

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

The PI3K/PTEN pathway plays a major role in carcinogenesis. Dysregulation of this pathway occurs frequently in breast cancer, and loss of PTEN expression is emerging as a potentially important mechanism of resistance to the widely used anti-HER2 therapy, trastuzumab. However, assays for loss of PTEN expression have suffered from lack of consistency. Here, we describe an automated and reliable protocol for PTEN protein expression by immunohistochemistry in formalin-fixed paraffin-embedded tissue that can be easily incorporated into clinical trials.

引用

页码：371 / 374

页数：4

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