Application of molecular probes for detecting foot-and-mouth disease virus

The aim of these studies was to prepare the digoxigenin (DIG)-labeled molecular probes for the identification of RT-PCR products. FMD infectious biological material from the laboratory collection was used. The PCR reaction of cDNA of all FMDV samples was performed using 30 cycles. The specificity of amplicons was confirmed by Southern Blot hybridization using DIG-labeled probes. By the use of DIG for the labeling of FMD cDNA it was possible to obtain very specific and sensitive molecular probes. The hybridization method allowed us to verify RT-PCR results and can be applied as an additional diagnostic method for FMD.