Chemistry Nobel Honors CRISPR-Cas9

被引：0

作者：

You Li-Lan ^{[1
,2
]}

Sun Wei ^{[1
]}

Yang Xiao-Qi ^{[1
,2
]}

Wang Yan-Li ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

来源：

PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS | 2020年 / 47卷 / 11期

基金：

中国国家自然科学基金;

关键词：

Nobel Prize in Chemistry; CRISPR-Cas9; gene-editing; RNA; REPEATS; GENE; IDENTIFICATION; ENDONUCLEASE; SEQUENCES; CLEAVAGE; IMMUNITY;

D O I：

10.16476/j.pibb.2020.0395

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Nobel Prize in Chemistry 2020 was awarded jointly to Emmanuelle Charpentier and Jennifer A. Doudna "for the development of CRISPR genome editing tool". They have discovered the sharpest tools for gene editing: the CRISPR/Cas9 genetic scissors, which have revolutionary impact on life sciences. The CRISPR system is an adaptive immune system in prokaryotes. Cas9, an effector protein of type. CRISPR-Cas system, functions as a dual-RNA-guided DNA endonuclease, which is able to cleave any dsDNA generating dsDNA break. The CRISPR-Cas9 has been widely used in gene editing, due to its simplicity, high efficiency and low price. This paper introduces the research findings of 2020 Chemistry Nobel Prize winners, summarizes the discovery process of CRISPR system and the activity and application of CRISPR-Cas9.

引用

页码：1119 / 1126

页数：8

共 38 条

[1] C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector
Abudayyeh, Omar O.
Gootenberg, Jonathan S.
Konermann, Silvana
Joung, Julia
Slaymaker, Ian M.
Cox, David B. T.
Shmakov, Sergey
Makarova, Kira S.
Semenova, Ekaterina
Minakhin, Leonid
Severinov, Konstantin
Regev, Aviv
Lander, Eric S.
Koonin, Eugene V.
Zhang, Feng
[J]. SCIENCE, 2016, 353 (6299)
[2] CRISPR provides acquired resistance against viruses in prokaryotes
Barrangou, Rodolphe
Fremaux, Christophe
Deveau, Helene
Richards, Melissa
Boyaval, Patrick
Moineau, Sylvain
Romero, Dennis A.
Horvath, Philippe
[J]. SCIENCE, 2007, 315 (5819) : 1709 - 1712
[3] Clustered regularly interspaced short palindrome repeats (CRISPRs) have spacers of extrachromosomal origin
Bolotin, A
Ouinquis, B
Sorokin, A
Ehrlich, SD
[J]. MICROBIOLOGY-SGM, 2005, 151 : 2551 - 2561
[4] Small CRISPR RNAs guide antiviral defense in prokaryotes
Brouns, Stan J. J.
Jore, Matthijs M.
Lundgren, Magnus
Westra, Edze R.
Slijkhuis, Rik J. H.
Snijders, Ambrosius P. L.
Dickman, Mark J.
Makarova, Kira S.
Koonin, Eugene V.
van der Oost, John
[J]. SCIENCE, 2008, 321 (5891) : 960 - 964
[5] Multiplex Genome Engineering Using CRISPR/Cas Systems
Cong, Le
Ran, F. Ann
Cox, David
Lin, Shuailiang
Barretto, Robert
Habib, Naomi
Hsu, Patrick D.
Wu, Xuebing
Jiang, Wenyan
Marraffini, Luciano A.
Zhang, Feng
[J]. SCIENCE, 2013, 339 (6121) : 819 - 823
[6] CRISPR gene editing tested in a person
Cyranoski, David
[J]. NATURE, 2016, 539 (7630) : 479 - 479
[7] CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III
Deltcheva, Elitza
Chylinski, Krzysztof
Sharma, Cynthia M.
Gonzales, Karine
Chao, Yanjie
Pirzada, Zaid A.
Eckert, Maria R.
Vogel, Joerg
Charpentier, Emmanuelle
[J]. NATURE, 2011, 471 (7340) : 602 - +
[8] The promise and challenge of therapeutic genome editing
Doudna, Jennifer A.
[J]. NATURE, 2020, 578 (7794) : 229 - 236
[9] The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA
Garneau, Josiane E.
Dupuis, Marie-Eve
Villion, Manuela
Romero, Dennis A.
Barrangou, Rodolphe
Boyaval, Patrick
Fremaux, Christophe
Horvath, Philippe
Magadan, Alfonso H.
Moineau, Sylvain
[J]. NATURE, 2010, 468 (7320) : 67 - 71
[10] Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria
Gasiunas, Giedrius
Barrangou, Rodolphe
Horvath, Philippe
Siksnys, Virginijus
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (39) : E2579 - E2586

← 1 2 3 4 →