In vitro androgenesis of triticale in isolated microspore culture

被引：40
|
作者：
Pauk, J
Puolimatka, M
Tóth, KL
Monostori, T
机构：
[1] Cereal Res Non Profit Co, Dept Wheat Genet & Breeding, H-6701 Szeged, Hungary
[2] Agr Res Ctr Finland, Plant breeding Res Sect, FIN-31600 Jokioinen, Finland
[3] St Stephen Univ, Dept Stat, H-2103 Godollo, Hungary
来源：
PLANT CELL TISSUE AND ORGAN CULTURE | 2000年 / 61卷 / 03期
基金：
匈牙利科学研究基金会;
关键词：
haploid plant; regeneration; sporophytic development; tissue culture; X Triticosecale;
D O I：
10.1023/A:1006416116366
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Culture conditions for triticale (X Triticosecale Wittmack) androgenesis were studied using microspore culture. Sporophytic development of isolated triticale microspores in culture is described in five winter hexaploid triticale genotypes. Microspores were isolated using a microblendor, and embryogenesis was induced in modified 190-2 medium both in the presence and absence of growth regulators. The highest induction of microspore embryogenesis was obtained in a growth regulator-free medium. Adventitious embryogenesis was observed during in vitro development of triticale microspores. Albino and green plantlets were regenerated from embryo-like structures. More than 50% of regenerants were albino. In total, 126 green plantlets were produced, transplanted and established in soil. Cytological evidence revealed that 90% of the transplanted regenerants were haploid.
引用
收藏
页码：221 / 229
页数：9
相关论文
共 50 条
  • [31] Effect of antioxidant on the regeneration of green plantlets in isolated microspore culture (IMC) derived doubled haploid in wheat and triticale
    Goyal, A.
    Bihari, L.
    Amundsen, E.
    Eudes, F.
    Randhawa, H. S.
    CANADIAN JOURNAL OF PLANT SCIENCE, 2012, 92 (03) : 609 - 609
  • [32] ANDROGENESIS IN ISOLATED MICROSPORES OF TRITICALE (XTRITICOSECALE WITTM.) INDUCED BY STRESS TREATMENTS
    Zur, I
    Dubas, E.
    Golemiec, E.
    Wedzony, M.
    ACTA PHYSIOLOGIAE PLANTARUM, 2004, 26 (03) : 140 - 140
  • [33] In vitro androgenesis in apple: Optimization of the anther culture
    Hofer, M
    HORTICULTURAL BIOTECHNOLOGY IN VITRO CULTURE AND BREEDING, 1997, (447): : 341 - 343
  • [34] Stress-induced changes important for effective androgenic induction in isolated microspore culture of triticale (XTriticosecale Wittm.)
    Zur, Iwona
    Dubas, Ewa
    Golemiec, Elzbieta
    Szechynska-Hebda, Magdalena
    Janowiak, Franciszek
    Wedzony, Maria
    PLANT CELL TISSUE AND ORGAN CULTURE, 2008, 94 (03) : 319 - 328
  • [35] Stress-induced changes important for effective androgenic induction in isolated microspore culture of triticale (×Triticosecale Wittm.)
    Iwona Żur
    Ewa Dubas
    Elżbieta Golemiec
    Magdalena Szechyńska-Hebda
    Franciszek Janowiak
    Maria Wędzony
    Plant Cell, Tissue and Organ Culture, 2008, 94 : 319 - 328
  • [36] First Stages of Microspore Reprogramming to Embryogenesis through Isolated Microspore Culture in Loquat
    Padoan, D.
    Chiancone, B.
    Germana, M. A.
    Khan, P. S. S. V.
    Barany, I.
    Risueno, M. C.
    Testillano, P. S.
    III INTERNATIONAL SYMPOSIUM ON LOQUAT, 2011, 887 : 285 - 290
  • [37] Variability of the Triticale Genome in Culture in vitro
    Pykalo, S. V.
    Dubrovna, O. V.
    CYTOLOGY AND GENETICS, 2018, 52 (05) : 385 - 393
  • [38] ANDROGENESIS IN PEARL-MILLET .2. ANALYSIS OF PLANTS OBTAINED FROM MICROSPORE CULTURE
    HA, DBD
    PERNES, J
    ZEITSCHRIFT FUR PFLANZENPHYSIOLOGIE, 1982, 108 (04): : 317 - 327
  • [39] Variability of the Triticale Genome in Culture in vitro
    S. V. Pykalo
    O. V. Dubrovna
    Cytology and Genetics, 2018, 52 : 385 - 393
  • [40] Influence of growth regulators level on the initial phases of androgenic induction in isolated microspore culture of triticale (x Triticosecale Wittm.)
    Zur, Iwona
    Dubas, Ewa
    Filek, Maria
    Biesaga-Koscielniak, Jolanta
    Golemiec, Elzbieta
    Wedzony, Maria
    ACTA BIOLOGICA CRACOVIENSIA SERIES BOTANICA, 2005, 47 : 91 - 91
12345