Biochemical Characteristics, Adhesion, and Cytotoxicity of Environmental and Clinical Isolates of Herbaspirillum spp.

被引:19
|
作者
Marques, Ana C. Q. [1 ]
Paludo, Katia S. [2 ]
Dallagassa, Cibelle B. [1 ]
Surek, Monica [1 ]
Pedrosa, Fabio O. [3 ]
Souza, Emanuel M. [3 ]
Cruz, Leonardo M. [3 ]
LiPuma, John J. [4 ]
Zanata, Slvio M. [5 ]
Rego, Fabiane G. M. [1 ]
Fadel-Picheth, Cyntia M. T. [1 ]
机构
[1] Univ Fed Parana, Dept Anal Clin, Setor Ciencias Saude, BR-80060000 Curitiba, Parana, Brazil
[2] Univ Estadual Ponta Grossa, Dept Biol Estrut Mol Genet, Ponta Grossa, Parana, Brazil
[3] Univ Fed Parana, Dept Bioquim & Biol Mol, Setor Ciencias Biol, BR-80060000 Curitiba, Parana, Brazil
[4] Univ Michigan, Dept Pediat, Ann Arbor, MI 48109 USA
[5] Univ Fed Parana, Dept Patol Basica, Setor Ciencias Biol, BR-80060000 Curitiba, Parana, Brazil
关键词
NITROGEN-FIXING BACTERIUM; ESCHERICHIA-COLI; GENOME SEQUENCE; SP-NOV; PSEUDOMONAS-RUBRISUBALBICANS; ACETOBACTER-DIAZOTROPHICUS; PATHOGENIC BACTERIA; PHASEOLUS-VULGARIS; MASS-SPECTROMETRY; ROOT-NODULES;
D O I
10.1128/JCM.02192-14
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Herbaspirillum bacteria are best known as plant growth-promoting rhizobacteria but have also been recovered from clinical samples. Here, biochemical tests, matrix-assisted laser deionization-time of flight (MALDI-TOF) mass spectrometry, adherence, and cytotoxicity to eukaryotic cells were used to compare clinical and environmental isolates of Herbaspirillum spp. Discrete biochemical differences were observed between human and environmental strains. All strains adhered to HeLa cells at low densities, and cytotoxic effects were discrete, supporting the view that Herbaspirillum bacteria are opportunists with low virulence potential.
引用
收藏
页码:302 / 308
页数:7
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