Structure-function studies of the hammerhead ribozyme

被引:18
|
作者
Verma, S [1 ]
Vaish, NK [1 ]
Eckstein, F [1 ]
机构
[1] Max Planck Inst Expt Med, D-37075 Gottingen, Germany
关键词
D O I
10.1016/S1367-5931(97)80049-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Elucidation of the catalytic mechanism and structure-function relationship studies of the hammerhead ribozyme continue to be an area of intensive research. A combination of diverse approaches, such as X ray crystallography, spectral studies, chemical modifications, sequence variations and kinetic analyses, have provided valuable insight into the cleavage mechanism of this ribozyme. The hammerhead ribozyme crystal structures have provided valuable insight into conformational deformations needed to attain the catalytically active structure. Similarly, determination of ribozyme solution structure by spectroscopic analyses and the effect of divalent metal ions on RNA folding has further aided in the construction of a model for hammermead catalysis.
引用
收藏
页码:532 / 536
页数:5
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