Periprosthetic joint infection with Ureaplasma parvum detected by 16S rRNA PCR

被引:3
|
作者
Ball, Nicola Diane [1 ]
Snape, Susan Elizabeth [1 ]
机构
[1] Nottingham Univ Hosp NHS Trust, Infect Dis, Nottingham, England
关键词
infections; bone and joint infections;
D O I
10.1136/bcr-2020-239858
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Usual culture methods to identify pathogenic bacteria can be unsuccessful, particularly when working with fastidious organisms. We present a case of early periprosthetic knee joint infection with Ureaplasma parvum only identified using 16S ribosomal RNA PCR. This case represents the impact molecular methods of bacterial identification can have on clinical care allowing for more targeted antimicrobial therapy; something which is imperative in an era of increasing antimicrobial resistance.
引用
收藏
页数:3
相关论文
共 50 条
  • [21] Bilateral periprosthetic joint infection with Ureaplasma urealyticum in an immunocompromised patient
    R. L. Roerdink
    C. M. Douw
    A. C. A. P. Leenders
    R. S. Dekker
    M. Dietvorst
    C. J. M. Oosterbos
    H. T. J. Roerdink
    R. W. T. M. Kempen
    L. P. A. Bom
    Infection, 2016, 44 : 807 - 810
  • [22] Bilateral periprosthetic joint infection with Ureaplasma urealyticum in an immunocompromised patient
    Roerdink, R. L.
    Douw, C. M.
    Leenders, A. C. A. P.
    Dekker, R. S.
    Dietvorst, M.
    Oosterbos, C. J. M.
    Roerdink, H. T. J.
    Kempen, R. W. T. M.
    Bom, L. P. A.
    INFECTION, 2016, 44 (06) : 807 - 810
  • [23] Evaluation of 16S rRNA Gene PCR Sensitivity and Specificity for Diagnosis of Prosthetic Joint Infection: a Prospective Multicenter Cross-Sectional Study
    Bemer, Pascale
    Plouzeau, Chloe
    Tande, Didier
    Leger, Julie
    Giraudeau, Bruno
    Valentin, Anne Sophie
    Jolivet-Gougeon, Anne
    Vincent, Pascal
    Corvec, Stephane
    Gibaud, Sophie
    Juvin, Marie Emmanuelle
    Hery-Arnaud, Genevieve
    Lemarie, Carole
    Kempf, Marie
    Bret, Laurent
    Quentin, Roland
    Coffre, Carine
    de Pinieux, Gonzague
    Bernard, Louis
    Burucoa, Christophe
    JOURNAL OF CLINICAL MICROBIOLOGY, 2014, 52 (10) : 3583 - 3589
  • [24] Phylogenetic Clustering of Soil Microbial Communities by 16S rRNA but Not 16S rRNA Genes
    DeAngelis, Kristen M.
    Firestone, Mary K.
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2012, 78 (07) : 2459 - 2461
  • [25] Bacterial taxa associated with the hematophagous mite Dermanyssus gallinae detected by 16S rRNA PCR amplification and TTGE fingerprinting
    Moro, Claire Valiente
    Thioulouse, Jean
    Chauve, Claude
    Normand, Philippe
    Zenner, Lionel
    RESEARCH IN MICROBIOLOGY, 2009, 160 (01) : 63 - 70
  • [26] Employment of broad-range 16S rRNA PCR to detect aetiological agents of infection from clinical specimens in patients with acute meningitis - rapid separation of 16S rRNA PCR amplicons without the need for cloning
    Xu, J
    Millar, BC
    Moore, JE
    Murphy, K
    Webb, H
    Fox, AJ
    Cafferkey, M
    Crowe, MJ
    JOURNAL OF APPLIED MICROBIOLOGY, 2003, 94 (02) : 197 - 206
  • [27] Evaluation of AMPLICOR Neisseria gonorrhoeae PCR using cppB nested PCR and 16S rRNA PCR
    Farrell, DJ
    JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (02) : 386 - 390
  • [28] Triplicate PCR reactions for 16S rRNA gene amplicon sequencing are unnecessary
    Marotz, Clarisse
    Sharma, Anukriti
    Humphrey, Greg
    Gottel, Neil
    Daum, Christopher
    Gilbert, Jack A.
    Eloe-Fadrosh, Emiley
    Knight, Rob
    BIOTECHNIQUES, 2019, 67 (01) : 29 - 32
  • [29] PCR primers targeting the 16S rRNA gene for the specific detection of streptomycetes
    Rintala, H
    Nevalainen, A
    Rönkä, E
    Suutari, M
    MOLECULAR AND CELLULAR PROBES, 2001, 15 (06) : 337 - 347