Optimized electroporation-induced transformation in Microcystis aeruginosa PCC7806

被引：0
|
作者：
El Semary, Nermin Adel [1 ]
机构：
[1] Helwan Univ, Fac Sci, Dept Bot & Microbiol, ET-11795 Helwan, Egypt
来源：
BIOTECHNOLOGIE AGRONOMIE SOCIETE ET ENVIRONNEMENT | 2010年 / 14卷 / 01期
关键词：
Gene disruption; homologous recombination; electroporation; transformation; Microcystis aeruginosa PCC7806; HPLC; CYANOBACTERIUM;
D O I：
暂无
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Gene disruption in cyanobacteria is difficult and comprises an obstacle for genetic manipulation. Very few reports tackled this problem but the methods used are usually obscure and hardly reproducible. Here we describe an optimized electroporation-induced transformation in Microcystis aeruginosa PCC7806 where conditions for successful electroporation and transformation are investigated.
引用
收藏
页码：149 / 152
页数：4
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