Efficient downregulation of alb1 gene using an AMA1-based episomal expression of RNAi construct in Aspergillus fumigatus

被引：15
|
作者：
Khalaj, Vahid
Eslami, Hamid
Azizi, Mohammad
Rovira-Graells, Nuria
Bromley, Mike
机构：
[1] Pasteur Inst Iran, Biotechnol Res Ctr, Tehran 13164, Iran
[2] F2g Ltd, Manchester, Lancs, England
来源：
FEMS MICROBIOLOGY LETTERS | 2007年 / 270卷 / 02期
关键词：
AMA1; RNAi; Aspergillus; cbhB;
D O I：
10.1111/j.1574-6968.2007.00680.x
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
An episomal RNAi silencing construct containing the inducible cbhB promoter and a hairpin structure has been made to downregulate the alb1 gene in the human pathogen Aspergillus fumigatus. Transformation of fungal protoplasts resulted in a high number of transformants with an inducible silenced phenotype (white spores). Efficient downregulation of the alb1 gene using this system suggests that this approach may overcome the variable downregulation observed with integrative constructs.
引用
收藏
页码：250 / 254
页数：5
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