Honeybee silk: Recombinant protein production, assembly and fiber spinning

被引：63
|
作者：
Weisman, Sarah [1 ]
Haritos, Victoria S. [1 ]
Church, Jeffrey S. [2 ]
Huson, Mickey G. [2 ]
Mudie, Stephen T. [3 ]
Rodgers, Andrew J. W. [4 ]
Dumsday, Geoff J. [4 ]
Sutherland, Tara D. [1 ]
机构：
[1] CSIRO Entomol, Acton, ACT 2601, Australia
[2] CSIRO Mat Sci & Engn, Belmont, Vic 3216, Australia
[3] CSIRO Mat Sci & Engn, Clayton, Vic 3168, Australia
[4] CSIRO Mol & Hlth Technol, Clayton, Vic 3168, Australia
来源：
BIOMATERIALS | 2010年 / 31卷 / 09期
关键词：
Biomimetic material; FTIR; Honeybee; Apis mellifera; Recombinant protein; Silk; IDENTIFICATION; SPIDER;
D O I：
10.1016/j.biomaterials.2009.12.021
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Transgenic production of silkworm and spider silks as biomaterials has posed intrinsic problems due to the large size and repetitive nature of the silk proteins In contrast the silk of honeybees (Apis mellifera) is composed of a family of four small and non-repetitive fibrous proteins We report recombinant production and purification of the four full-length unmodified honeybee silk proteins in Escherichia colt at substantial yields of 0 2-2 5 g/L. Under the correct conditions the recombinant proteins self-assembled to reproduce the native coiled coil structure. Using a simple biomimetic spinning system we could fabricate recombinant silk fibers that replicated the tensile strength of the native material (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码：2695 / 2700
页数：6
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