Replication of the Plasmodium falciparum apicoplast DNA initiates within the inverted repeat region

被引：21
|
作者：
Singh, D [1 ]
Chaubey, S [1 ]
Habib, S [1 ]
机构：
[1] Cent Drug Res Inst, Mol & Struct Biol Div, Lucknow 226001, Uttar Pradesh, India
来源：
MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 2003年 / 126卷 / 01期
关键词：
Plasmodium falciparum; apicoplast; replication origin; inverted repeat;
D O I：
10.1016/S0166-6851(02)00251-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The 35 kb apicoplast genomes (pIDNA) of Plasmodium,falciparum and Toxoplasma gondii share close sequence similarity but differ in their in vivo topologies. Although sequence analysis of tandem repeats of T gondii pIDNA has suggested the presence of replication initiation sites within the inverted repeat region, the replication origins (ori) of the P.falciparum circular pIDNA have not been identified. Using 5' end-labelled nascent DNA as probe, we demonstrate that the ori of P.falciparum pIDNA is localised within the inverted repeat region. Our results also indicate the presence of two initiation sites within each inverted repeat segment of the circular pIDNA of P fialcipartim suggestive of a four D-loop/bi-directional ori mechanism of DNA replication. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：9 / 14
页数：6
相关论文
共 50 条
  • [31] Altered expression of K13 disrupts DNA replication and repair in Plasmodium falciparum
    Justin Gibbons
    Katrina A. Button-Simons
    Swamy R. Adapa
    Suzanne Li
    Maxwell Pietsch
    Min Zhang
    Xiangyun Liao
    John H. Adams
    Michael T. Ferdig
    Rays H. Y. Jiang
    BMC Genomics, 19
  • [32] Altered expression of K13 disrupts DNA replication and repair in Plasmodium falciparum
    Gibbons, Justin
    Button-Simons, Katrina A.
    Adapa, Swamy R.
    Li, Suzanne
    Pietsch, Maxwell
    Zhang, Min
    Liao, Xiangyun
    Adams, John H.
    Ferdig, Michael T.
    Jiang, Rays H. Y.
    BMC GENOMICS, 2018, 19
  • [33] REPLICATION INITIATES AT A CONFINED REGION DURING DNA AMPLIFICATION IN SCIARA DNA PUFF II-9A
    LIANG, C
    SPITZER, JD
    SMITH, HS
    GERBI, SA
    GENES & DEVELOPMENT, 1993, 7 (06) : 1072 - 1084
  • [34] Single-stranded DNA binding protein from human malarial parasite Plasmodium falciparum is encoded in the nucleus and targeted to the apicoplast
    Prusty, Dhaneswar
    Dar, Ashraf
    Priya, Rashmi
    Sharma, Atul
    Dana, Srikanta
    Choudhury, Nirupam Roy
    Rao, N. Subba
    Dhar, Suman Kumar
    NUCLEIC ACIDS RESEARCH, 2010, 38 (20) : 7037 - 7053
  • [35] Plasmodium falciparum CRK4 directs continuous rounds of DNA replication during schizogony
    Markus Ganter
    Jonathan M. Goldberg
    Jeffrey D. Dvorin
    Joao A. Paulo
    Jonas G. King
    Abhai K. Tripathi
    Aditya S. Paul
    Jing Yang
    Isabelle Coppens
    Rays H. Y. Jiang
    Brendan Elsworth
    David A. Baker
    Rhoel R. Dinglasan
    Steven P. Gygi
    Manoj T. Duraisingh
    Nature Microbiology, 2
  • [36] Unconventional trafficking of a DNA replication protein through secretory pathway in apicomplexan Plasmodium falciparum.
    Sharma, B.
    Sharma, R.
    Dhar, S. K.
    MOLECULAR BIOLOGY OF THE CELL, 2017, 28
  • [37] Force Spectroscopy of the Plasmodium falciparum Vaccine Candidate Circumsporozoite Protein Suggests a Mechanically Pliable Repeat Region
    Patra, Aditya Prasad
    Sharma, Shobhona
    Ainavarapu, Rama Koti
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2017, 292 (06) : 2110 - 2119
  • [38] DNA replication during intra-erythrocytic stages of human malarial parasite Plasmodium falciparum
    Mitra, Pallabi
    Deshmukh, Abhijit S.
    Dhar, Suman Kumar
    CURRENT SCIENCE, 2012, 102 (05): : 725 - 740
  • [39] Chelation of Ca2+ ions by a peptide from the repeat region of the Plasmodium falciparum circumsporozoite protein
    Elena Topchiy
    Teresa Lehmann
    Malaria Journal, 13
  • [40] Chelation of Ca2+ ions by a peptide from the repeat region of the Plasmodium falciparum circumsporozoite protein
    Topchiy, Elena
    Lehmann, Teresa
    MALARIA JOURNAL, 2014, 13
12345