Improving aptamer performance with nucleic acid mimics: de novo and post-SELEX approaches

被引：33

作者：

Oliveira, Ricardo ^{[1
,2
]}

Pinho, Eva ^{[1
]}

Sousa, Ana Luisa ^{[1
,2
]}

DeStefano, Jeffrey J. ^{[3
]}

Azevedo, Nuno Filipe ^{[2
]}

Almeida, Carina ^{[1
,2
,4
]}

机构：

[1] INIAV Natl Inst Agr & Veterinarian Res, Rua Lagidos, P-4485655 Vairao, Vila Do Conde, Portugal

[2] Univ Porto, Fac Engn, LEPABE Lab Proc Engn Environm Biotechnol & Energy, Rua Dr Roberto Frias, P-4200465 Porto, Portugal

[3] Univ Maryland, Cell Biol & Mol Genet, Biosci Res Bldg, College Pk, MD 20742 USA

[4] Univ Minho, Ctr Biol Engn, Campus Gualtar, P-4710057 Braga, Portugal

来源：

TRENDS IN BIOTECHNOLOGY | 2022年 / 40卷 / 05期

关键词：

THROMBIN BINDING APTAMER; G-QUADRUPLEX; HIGH-AFFINITY; DNA APTAMERS; TAR RNA; CHEMICAL-MODIFICATIONS; VASCULAR-PERMEABILITY; VEGF APTAMER; STABILITY; SELECTION;

D O I：

10.1016/j.tibtech.2021.09.011

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aptamers are structural single-stranded oligonucleotides generated in vitro to bind to a specific target molecule. Aptamers' versatility can be enhanced with nucleic acid mimics (NAMs) during or after a selection process, also known as systematic evolution of ligands by exponential enrichment (SELEX). We address advantages and limitations of the technologies used to generate NAM aptamers, especially the applicability of existing engineered polymerases to replicate NAMs and methodologies to improve aptamers after SELEX. We also discuss the limitations of existing methods for sequencing NAM sequences and bioinformatic tools to predict NAM aptamer structures. As a conclusion, we suggest that NAM aptamers might successfully compete with molecular tools based on proteins such as antibodies for future application.

引用

页码：549 / 563

页数：15

共 42 条

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