Reactive cysteine is protonated in the triplet excited state of the LOV2 domain in Adiantum phytochrome3

被引：99
|
作者：
Sato, Y
Iwata, T
Tokutomi, S
Kandori, H [1 ]
机构：
[1] Nagoya Inst Technol, Dept Mat Sci & Engn, Showa Ku, Nagoya, Aichi 4668555, Japan
[2] Osaka Prefecture Univ, Adv Sci & Technol Res Inst, Osaka 5998570, Japan
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2005年 / 127卷 / 04期
关键词：
D O I：
10.1021/ja0436897
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Phototropin is a plant blue-light sensor protein that possesses a flavin mononucleotide (FMN) as the chromophore in LOV domains. Its photoreaction is an adduct formation between FMN and a nearby cysteine that takes place in the triplet excited state of FMN. In this communication, we revealed that the reactive cysteine is protonated in the triplet excited state of the LOV2 domain of Adiantum phytochrome3 by means of low-temperature FTIR spectroscopy. Its hydrogen-bonding interaction is strengthened in the triplet excited state, presumably with the FMN chromophore. Such strong interaction drives adduct formation on a microsecond time scale. Copyright © 2005 American Chemical Society.
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收藏
页码：1088 / 1089
页数：2
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