Role of Fks1p and Matrix Glucan in Candida albicans Biofilm Resistance to an Echinocandin, Pyrimidine, and Polyene

被引：148
|
作者：
Nett, Jeniel E. [1 ,3 ]
Crawford, Kyler [1 ]
Marchillo, Karen [1 ]
Andes, David R. [1 ,2 ]
机构：
[1] Univ Wisconsin, Dept Med, Madison, WI 53792 USA
[2] Univ Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53792 USA
[3] Univ Wisconsin, Dept Cellular & Mol Biol, Madison, WI 53792 USA
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2010年 / 54卷 / 08期
基金：
美国国家卫生研究院;
关键词：
IN-VITRO; FLUCONAZOLE RESISTANCE; ANTIFUNGAL AGENTS; DRUG-RESISTANCE; EFFLUX PUMPS; IDENTIFICATION; GENE;
D O I：
10.1128/AAC.00227-10
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Candida infections frequently involve drug-resistant biofilm growth on device surfaces. Glucan synthase gene FKS1 has been linked to triazole resistance in Candida biofilms. We tested the impact of FKS1 modulation on susceptibility to additional antifungal classes. Reduction of FKS1 expression rendered biofilms more susceptible to amphotericin B, anidulafungin, and flucytosine. Increased resistance to anidulafungin and amphotericin B was observed for biofilms overexpressing FKS1. These findings suggest that Candida biofilm glucan sequestration is a multidrug resistance mechanism.
引用
收藏
页码：3505 / 3508
页数：4
相关论文
共 50 条
  • [31] Genetic Basis of Candida Biofilm Resistance Due to Drug-Sequestering Matrix Glucan
    Nett, Jeniel E.
    Sanchez, Hiram
    Cain, Michael T.
    Andes, David R.
    JOURNAL OF INFECTIOUS DISEASES, 2010, 202 (01): : 171 - 175
  • [32] A Candida Biofilm-Induced Pathway for Matrix Glucan Delivery: Implications for Drug Resistance
    Taff, Heather T.
    Nett, Jeniel E.
    Zarnowski, Robert
    Ross, Kelly M.
    Sanchez, Hiram
    Cain, Mike T.
    Hamaker, Jessica
    Mitchell, Aaron P.
    Andes, David R.
    PLOS PATHOGENS, 2012, 8 (08)
  • [33] Candida parapsilosis isolates carrying mutations outside FKS1 hotspot regions confer high echinocandin tolerance and facilitate the development of echinocandin resistance
    Daneshnia, Farnaz
    Arastehfar, Amir
    Lombardi, Lisa
    Binder, Ulrike
    Scheler, Jakob
    Shahandashti, Roya Vahedi
    Hagen, Ferry
    Lass-Floerl, Cornelia
    Mansour, Michael K.
    Butler, Geraldine
    Perlin, David S.
    INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2023, 62 (01)
  • [34] Effect of Candida glabrata FKS1 and FKS2 Mutations on Echinocandin Sensitivity and Kinetics of 1,3-β-D-Glucan Synthase: Implication for the Existing Susceptibility Breakpoint
    Garcia-Effron, Guillermo
    Lee, Samuel
    Park, Steven
    Cleary, John D.
    Perlin, David S.
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2009, 53 (09) : 3690 - 3699
  • [35] Identification of the FKS1 gene of Candida albicans as the essential target of 1,3-beta-D-glucan synthase inhibitors
    Douglas, CM
    DIppolito, JA
    Shei, GJ
    Meinz, M
    Onishi, J
    Marrinan, JA
    Li, W
    Abruzzo, GK
    Flattery, A
    Bartizal, K
    Mitchell, A
    Kurtz, MB
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1997, 41 (11) : 2471 - 2479
  • [36] Breakthrough fungemia due to Candida fermentati with fks1p mutation under micafungin treatment in a cord blood transplant recipient
    Konuma, Takaaki
    Takahashi, Shunsuke
    Kiyuna, Tomohiko
    Miharu, Yuta
    Suzuki, Masato
    Shibata, Hiroko
    Kato, Seiko
    Takahashi, Satoshi
    Tojo, Arinobu
    TRANSPLANT INFECTIOUS DISEASE, 2017, 19 (01)
  • [37] CaspofunginAcquired echinocandin resistance in Candida tropicalis due to S645P mutation in the HS-1 region of FKS1: case report
    Reactions Weekly, 2018, 1701 (1) : 80 - 80
  • [38] Role of SFP1 in the Regulation of Candida albicans Biofilm Formation
    Chen, Hsueh-Fen
    Lan, Chung-Yu
    PLOS ONE, 2015, 10 (06):
  • [39] Clinically significant micafungin resistance in Candida albicans involves modification of a glucan synthase catalytic subunit GSC1 (FKS1) allele followed by loss of heterozygosity
    Niimi, K.
    Monk, B. C.
    Hirai, A.
    Hatakenaka, K.
    Umeyama, T.
    Lamping, E.
    Maki, K.
    Tanabe, K.
    Kamimura, T.
    Ikeda, F.
    Uehara, Y.
    Kano, R.
    Hasegawa, A.
    Cannon, R. D.
    Niimi, M.
    JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2010, 65 (05) : 842 - 852
  • [40] Role for Fks1 in the Intrinsic Echinocandin Resistance of Fusarium solani as Evidenced by Hybrid Expression in Saccharomyces cerevisiae
    Katiyar, Santosh K.
    Edlind, Thomas D.
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2009, 53 (05) : 1772 - 1778
12345